Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea

Simple item page

Simple item page

Full item details

dc.contributor.author
McLoughlin, Austein G.
Wytinck, Nick
Walker, Philip L.
Girard, Ian J.
Rashid, Khalid Y.
De Kievit, Teresa
Fernando, W. G. Dilantha
Whyard, Steve
Belmonte, Mark F.
dc.date.accepted
2018-04-16
dc.date.accessioned
2024-07-03T12:30:43Z
dc.date.available
2024-07-03T12:30:43Z
dc.date.issued
2018-05-09
dc.date.submitted
2017-10-03
dc.description.abstract - en
Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S. sclerotiorum infection is becoming clearer, genetic control of the pathogen has been elusive and effective control of pathogen colonization using traditional broad-spectrum agro-chemical protocols are less effective than desired. In the current study, we developed species-specific RNA interference-based control treatments capable of reducing fungal infection. Development of a target identification pipeline using global RNA sequencing data for selection and application of double stranded RNA (dsRNA) molecules identified single gene targets of the fungus. Using this approach, we demonstrate the utility of this technology through foliar applications of dsRNAs to the leaf surface that significantly decreased fungal infection and S. sclerotiorum disease symptoms. Select target gene homologs were also tested in the closely related species, Botrytis cinerea, reducing lesion size and providing compelling evidence of the adaptability and flexibility of this technology in protecting plants against devastating fungal pathogens.
dc.identifier.citation
McLoughlin, A. G., Wytinck, N., Walker, P. L., Girard, I. J., Rashid, K. Y., de Kievit, T., Fernando, W. G., Whyard, S., & Belmonte, M. F. (2018). Identification and application of exogenous dsrna confers plant protection against sclerotinia sclerotiorum and botrytis cinerea. Scientific Reports, 8(1), Article 7320. https://doi.org/10.1038/s41598-018-25434-4
dc.identifier.doi
https://doi.org/10.1038/s41598-018-25434-4
dc.identifier.uri
https://open-science.canada.ca/handle/123456789/2648
dc.language.iso
en
dc.publisher
Nature Publishing Group
dc.rights - en
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.openaccesslevel - en
Gold
dc.rights.openaccesslevel - fr
Or
dc.rights.uri - en
https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr
https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en
Agriculture
dc.subject - fr
Agriculture
dc.subject.en - en
Agriculture
dc.subject.fr - fr
Agriculture
dc.title - en
Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea
dc.type - en
Article
dc.type - fr
Article
local.acceptedmanuscript.articlenum
7320
local.article.journalissue
1
local.article.journaltitle
Scientific Reports
local.article.journalvolume
8
local.peerreview - en
Yes
local.peerreview - fr
Oui
Download(s)

Original bundle

Now showing 1 - 1 of 1

Thumbnail image

Name: IdentificationandapplicationofexogenousdsRNA_2018.pdf

Size: 3.14 MB

Format: PDF

Download file

Collection(s)

Page details

Date modified: