Culture‑based determination of verticillium densities in soil overestimates disease pressure of verticillium wilt of potato in Manitoba
Culture‑based determination of verticillium densities in soil overestimates disease pressure of verticillium wilt of potato in Manitoba
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Full item details
- creativework.keywords - en
- propagule density
- real-time PCR
- verticillium wilt diseases
- potatoes
- creativework.keywords - fr
- pomme de terre
- verticillioses
- densité des propagules
- PCR en temps réel
- dc.contributor.author
- Molina, Oscar I.
- Henriquez, Maria A.
- Tenuta, Mario
- dc.date.accepted
- 2023-07-09
- dc.date.accessioned
- 2024-12-09T22:31:20Z
- dc.date.available
- 2024-12-09T22:31:20Z
- dc.date.issued
- 2023-08-27
- dc.description.abstract - en
- Verticillium wilt affects potato fields in Manitoba where 80% of the fields are planted to the moderately susceptible cultivar, Russet Burbank. An accurate determination of Verticillium dahliae inoculum in soil is critical for disease management. In this study, we investigated the presence of microsclerotia-producing Verticillium species in potato fields in Manitoba and compared published quantitative real-time PCR assays for V. dahliae, V. tricorpus and V. longisporum against a traditional plating method. Selected real-time PCR assays could differentiate and quantify the major microsclerotia-producing species, V. dahliae, V. tricorpus and V. longisporum. Results showed that the presence of V. tricorpus caused an overestimation of V. dahliae propagule density when using the plating method. As a result, Verticillium wilt severity was negatively related to cfu from traditional plating, while positive with the amounts of V. dahliae genomic DNA in soils.
- dc.identifier.citation
- Molina, O. I., Henriquez, M. A., & Tenuta, M. (2023). Culture-based determination of verticillium densities in soil overestimates disease pressure of verticillium wilt of potato in Manitoba. American Journal of Potato Research, 100, 324–339. https://doi.org/10.1007/s12230-023-09922-6
- dc.identifier.doi
- https://doi.org/10.1007/s12230-023-09922-6
- dc.identifier.issn
- 1099-209X
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3184
- dc.language.iso
- en
- dc.publisher - en
- Springer
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Agriculture
- Potatoes
- Plant diseases
- dc.subject - fr
- Agriculture
- Pomme de terre
- Maladie des plantes
- dc.subject.en - en
- Agriculture
- Potatoes
- Plant diseases
- dc.subject.fr - fr
- Agriculture
- Pomme de terre
- Maladie des plantes
- dc.title - en
- Culture‑based determination of verticillium densities in soil overestimates disease pressure of verticillium wilt of potato in Manitoba
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journaltitle - en
- American Journal of Potato Research
- local.article.journalvolume
- 100
- local.pagination
- 324-339
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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