Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk

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creativework.keywords - en
Transmissible spongiform encephalopathies
Chronic wasting disease--Diagnosis
creativework.keywords - fr
Encéphalopathies spongiformes transmissibles
Maladie du dépérissement chronique--Diagnostic
dc.contributor.author
Haley, N. J.
Donner, R.
Henderson, D. M.
Tennant, J.
Hoover, E. A.
Manca, M.
Caughey, B.
Kondru, N.
Manne, S.
Kanthasamay, A.
Hannaoui, S.
Chang, S. C.
Gilch, S.
Smiley, S.
Mitchell, G.
Lehmkuhl, A. D.
Thomsen, B. V.
dc.date.accepted
2020-01-10
dc.date.accessioned
2025-04-04T18:52:39Z
dc.date.available
2025-04-04T18:52:39Z
dc.date.issued
2020-01-23
dc.date.submitted
2019-10-09
dc.description.abstract - en
Chronic wasting disease is a progressively fatal, horizontally transmissible prion disease affecting several members of the cervid species. Conventional diagnosis relies on ELISA or IHC evaluation using tissues collected post-mortem; however, recent research has focused on newly developed amplification techniques using samples collected antemortem. The present study sought to cross-validate the real-time quaking-induced conversion assay (RT-QuIC) evaluation of rectal biopsies collected from an elk herd with endemic CWD, assessing both binary positive/negative test results as well as relative rates of amplification between laboratories. We found that results were correlative in both categories across all laboratories performing RT-QuIC, as well as to conventional IHC performed at a national reference laboratory. A significantly higher number of positive samples were identified using RT-QuIC, with results seemingly unhindered by low follicle counts. These findings support the continued development and implementation of amplification assays in the diagnosis of prion diseases of veterinary importance, targeting not just antemortem sampling strategies, but post-mortem testing approaches as well.
dc.identifier.citation
Haley, N. J., Donner, R., Henderson, D. M., Tennant, J., Hoover, E. A., Manca, M., Caughey, B., Kondru, N., Manne, S., Kanthasamay, A., Hannaoui, S., Chang, S. C., Gilch, S., Smiley, S., Mitchell, G., Lehmkuhl, A. D., & Thomsen, B. V. (2020). Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk. Prion, 14(1), 47–55. https://doi.org/10.1080/19336896.2020.1716657
dc.identifier.doi
https://doi.org/10.1080/19336896.2020.1716657
dc.identifier.issn
1933-690X
dc.identifier.uri
https://open-science.canada.ca/handle/123456789/3566
dc.language.iso
en
dc.publisher - en
Taylor & Francis
dc.publisher - fr
Taylor & Francis
dc.rights - en
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.uri - en
https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr
https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en
Animal diseases
Diagnosis
dc.subject - fr
Maladie animale
Diagnostic
dc.subject.en - en
Animal diseases
Diagnosis
dc.subject.fr - fr
Maladie animale
Diagnostic
dc.title - en
Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk
dc.type - en
Article
dc.type - fr
Article
local.article.journalissue
1
local.article.journaltitle - en
Prion
local.article.journalvolume
14
local.pagination
47-55
local.peerreview - en
Yes
local.peerreview - fr
Oui
local.requestdoi
No
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