Combining a universal capture ligand and pan-serotype monoclonal antibody to develop a pan-serotype lateral flow strip test for foot-and-mouth disease virus detection
Combining a universal capture ligand and pan-serotype monoclonal antibody to develop a pan-serotype lateral flow strip test for foot-and-mouth disease virus detection
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Full item details
- creativework.keywords - en
- Foot and mouth disease
- Foot-and-mouth disease virus
- Monoclonal antibodies
- Ligands
- creativework.keywords - fr
- Fièvre aphteuse
- Aphthovirus
- Anticorps monoclonaux
- Ligands
- dc.contributor.author
- Yang, Ming
- Zhmendak, Dmytro
- Mioulet, Valerie
- King, Donald P.
- Burman, Alison
- Nfon, Charles K.
- dc.date.accepted
- 2022-04-04
- dc.date.accessioned
- 2025-02-21T19:26:36Z
- dc.date.available
- 2025-02-21T19:26:36Z
- dc.date.issued
- 2022-04-10
- dc.date.submitted
- 2022-03-11
- dc.description.abstract - en
- Foot-and-mouth disease virus (FMDV) causes FMD, a highly contagious disease of cloven-hoofed animals including cattle, goats, pigs and sheep. Rapid detection of FMDV is critical to limit the devastating economic losses due to FMD. Current laboratory methods for FMDV detection such as virus isolation, real-time reverse transcription PCR and antigen detection enzyme-linked immunosorbent assay (AgELISA) are labor-intensive, requiring trained personnel and specialized equipment. We present the development and validation of a pan-serotype lateral flow strip test (LFST) that uses recombinant bovine integrin αvβ6 as a universal capture ligand and a pan-serotype monoclonal antibody (mAb) to detect FMDV. The LFST detected all seven FMDV serotypes, where the diagnostic sensitivity was comparable to the AgELISA, and the diagnostic specificity was 100% without cross-reactivity to other viruses causing vesicular disease in livestock. This rapid test will be useful for on-site FMDV detection, as well as in laboratories in endemic countries where laboratory resources are limited.
- dc.description.fosrcfull - en
- This article belongs to the Special Issue: Global Foot-and-Mouth Disease Control.
- dc.description.fosrcfull-fosrctranslation - fr
- Cet article fait partie du numéro spécial: Global Foot-and-Mouth Disease Control.
- dc.identifier.citation
- Yang, M., Zhmendak, D., Mioulet, V., King, D. P., Burman, A., & Nfon, C. K. (2022). Combining a universal capture ligand and pan-serotype monoclonal antibody to develop a pan-serotype lateral flow strip test for foot-and-mouth disease virus detection. Viruses, 14(4), Article 785. https://doi.org/10.3390/v14040785
- dc.identifier.doi
- https://doi.org/10.3390/v14040785
- dc.identifier.issn
- 1999-4915
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3457
- dc.language.iso
- en
- dc.publisher - en
- MDPI
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Animal diseases
- Viruses
- dc.subject - fr
- Maladie animale
- Virus
- dc.subject.en - en
- Animal diseases
- Viruses
- dc.subject.fr - fr
- Maladie animale
- Virus
- dc.title - en
- Combining a universal capture ligand and pan-serotype monoclonal antibody to develop a pan-serotype lateral flow strip test for foot-and-mouth disease virus detection
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 785
- local.article.journalissue
- 4
- local.article.journaltitle - en
- Viruses
- local.article.journalvolume
- 14
- local.pagination
- 1-8
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
- local.requestdoi
- No
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