A scalable serology solution for profiling humoral immune responses to SARS-CoV-2 infection and vaccination
A scalable serology solution for profiling humoral immune responses to SARS-CoV-2 infection and vaccination
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- dc.contributor.author
- Colwill, Karen
- Arnold, Corey
- Rathod, Bhavisha
- Abe, Kento T.
- Wang, Jenny H.
- Pasculescu, Adrian
- Maltseva, Mariam
- Rocheleau, Lynda
- Pelchat, Martin
- Fazel-Zarandi, Mahya
- Iskilova, Mariam
- Barrios-Rodiles, Miriam
- Bennett, Linda
- Yau, Kevin
- Cholette, François
- Mesa, Christine
- Li, Angel X.
- Paterson, Aimee
- Hladunewich, Michelle A.
- Goodwin, Pamela J.
- Wrana, Jeffrey L.
- Drews, Steven J.
- Mubareka, Samira
- McGeer, Allison J.
- Kim, John
- Langlois, Marc-André
- Gingras, Anne-Claude
- Durocher, Yves
- Langlois, Marc-Andre
- Galipeau, Yannick
- Stuible, Matthew
- dc.date.accessioned
- 2024-11-18T16:07:51Z
- dc.date.available
- 2024-11-18T16:07:51Z
- dc.date.issued
- 2022-03-23
- dc.description.abstract - en
- OBJECTIVES: Antibody testing against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in detecting previous exposures and analyzing vaccine-elicited immune responses. Here, we describe a scalable solution to detect and quantify SARS-CoV-2 antibodies, discriminate between natural infection- and vaccination-induced responses, and assess antibody-mediated inhibition of the spike-angiotensin converting enzyme 2 (ACE2) interaction. METHODS: We developed methods and reagents to detect SARS-CoV-2 antibodies by enzyme-linked immunosorbent assay (ELISA). The main assays focus on the parallel detection of immunoglobulin (Ig)Gs against the spike trimer, its receptor binding domain (RBD) and nucleocapsid (N). We automated a surrogate neutralisation (sn)ELISA that measures inhibition of ACE2-spike or -RBD interactions by antibodies. The assays were calibrated to a World Health Organization reference standard. RESULTS: Our single-point IgG-based ELISAs accurately distinguished non-infected and infected individuals. For seroprevalence assessment (in a non-vaccinated cohort), classifying a sample as positive if antibodies were detected for ≥ 2 of the 3 antigens provided the highest specificity. In vaccinated cohorts, increases in anti-spike and -RBD (but not -N) antibodies are observed. We present detailed protocols for serum/plasma or dried blood spots analysis performed manually and on automated platforms. The snELISA can be performed automatically at single points, increasing its scalability. CONCLUSIONS: Measuring antibodies to three viral antigens and identify neutralising antibodies capable of disrupting spike-ACE2 interactions in high-throughput enables large-scale analyses of humoral immune responses to SARS-CoV-2 infection and vaccination. The reagents are available to enable scaling up of standardised serological assays, permitting inter-laboratory data comparison and aggregation.
- dc.identifier.doi
- https://doi.org/10.1002/cti2.1380
- dc.identifier.issn
- 2050-0068
- dc.identifier.pubmedID
- 35356067
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3160
- dc.language.iso
- en
- dc.publisher - en
- Australian and New Zealand Society for Immunology
- dc.rights - en
- Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
- dc.rights - fr
- Creative Commons Attribution-Pas d’Utilisation Commerciale 4.0 International (CC BY-NC 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by-nc/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by-nc/4.0/deed.fr
- dc.subject - en
- Health
- Coronavirus diseases
- Screening (Medicine)
- dc.subject - fr
- Santé
- Maladie à coronavirus
- Dépistage (Médecine)
- dc.subject.en - en
- Health
- Coronavirus diseases
- Screening (Medicine)
- dc.subject.fr - fr
- Santé
- Maladie à coronavirus
- Dépistage (Médecine)
- dc.title - en
- A scalable serology solution for profiling humoral immune responses to SARS-CoV-2 infection and vaccination
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- E1380
- local.article.journaltitle - en
- Clinical & Translational Immunology
- local.article.journalvolume
- 11
- local.pagination
- 1-22
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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