Microsatellite mutation frequencies in river otters (Lontra Canadensis) from the Athabasca Oil Sands region are correlated to polycyclic aromatic compound tissue burden

Gyasi, Helina; Curry, Jory; Browning, Jared; Ha, Kelsey; Thomas, Philippe J.; O'Brien, Jason M.

doi:https://open-science.canada.ca/handle/123456789/2651

Microsatellite mutation frequencies in river otters (Lontra Canadensis) from the Athabasca Oil Sands region are correlated to polycyclic aromatic compound tissue burden

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dc.contributor.author: Gyasi, Helina; Curry, Jory; Browning, Jared; Ha, Kelsey; Thomas, Philippe J.; O'Brien, Jason M.
dc.date.accepted: 2022-04-22
dc.date.accessioned: 2024-07-04T17:53:28Z
dc.date.available: 2024-07-04T17:53:28Z
dc.date.issued: 2022-04-22
dc.date.submitted: 2022-02-24
dc.description.abstract - en: Mining activities in the Athabasca oil sands region (AOSR) have contributed to an increase of polycyclic aromatic compounds (PACs) locally. However, many PACs found in the AOSR, and the combined effects of PAC mixtures have not been evaluated for genotoxicity in wildlife. Here, we examine whether mutation frequencies in AOSR river otters are correlated to PAC tissue burdens. We used single-molecule polymerase chain reaction (SM-PCR) to measure the mutant frequency of unstable DNA microsatellite loci in the bone marrow of wild river otters (n=11) from the AOSR. Microsatellite mutation frequencies were regressed against liver PAC burden (total, low/high molecular weight (LMW/HMW), and parent/alkylated PACs), and to the distances from where the samples were collected to nearby bitumen upgraders. We found that microsatellite mutation frequency was positively correlated with total liver PAC burden. LMW and alkylated PACs were detected at higher levels and had a stronger positive relationship with mutation frequency than HMW (alkylated and parent) PACs. There were no significant relationships detected between mutation frequency and LMW parent PACs or the distance from bitumen upgraders. Furthermore, pyrogenic and petrogenic signatures suggest PACs in animals with high mutation frequencies were associated with combustion processes; although further investigation is warranted, due to limitations of diagnostic ratio determination with biotic models. Our findings support the hypothesis that PACs found in the AOSR increase mutation frequency in wildlife. Further investigation is required to determine if the elevated PAC levels associated with higher mutation frequency are due to natural exposure or elevated human activity.
dc.description.fosrcfull - en: This is the peer reviewed version of the following article: Gyasi, H., Curry, J., Browning, J., Ha, K., Thomas, P.J. & O'Brien, J.M. (2022) Microsatellite mutation frequencies in river otters (Lontra Canadensis) from the Athabasca Oil Sands region are correlated to polycyclic aromatic compound tissue burden. Environmental and Molecular Mutagenesis, 63(4), 172–183. Available from: https://doi.org/10.1002/em.22482, which has been published in final form at <a href="https://doi.org/10.1002/em.22482">https://doi.org/10.1002/em.22482</a> . This article may be used for non-commercial purposes in accordance with <a href="https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html">Wiley Terms and Conditions for Use of Self-Archived Versions,</a>. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
dc.description.fosrcfull-fosrctranslation - fr: Ceci est la version révisée par les pairs de l'article suivant : Gyasi, H., Curry, J., Browning, J., Ha, K., Thomas, P.J. & O'Brien, J.M. (2022) Microsatellite mutation frequencies in river otters (Lontra Canadensis) from the Athabasca Oil Sands region are correlated to polycyclic aromatic compound tissue burden. Environmental and Molecular Mutagenesis, 63(4), 172–183. Available from: https://doi.org/10.1002/em.22482, qui a été publié sous sa forme finale à <a href="https://doi.org/10.1002/em.22482">https://doi.org/10.1002/em.22482</a>. Cet article peut être utilisé à des fins non commerciales conformément aux <a href="https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html">conditions générales d'utilisation des versions auto-archivées de Wiley</a> (en anglais seulement). Cet article ne peut être amélioré, enrichi ou transformé en une œuvre dérivée sans l'autorisation formelle de Wiley ou sans les droits prévus par la législation en vigueur. Les avis de droit d'auteur ne doivent pas être supprimés, masqués ou modifiés. L'article doit être lié à la version publiée de Wiley sur Wiley Online Library et il est interdit à des tiers d'incorporer, d'encadrer ou de mettre à disposition l'article ou des pages de celui-ci à partir de plates-formes, de services et de sites web autres que Wiley Online Library.
dc.identifier.issn: 1098-2280; 0893-6692
dc.identifier.uri: https://open-science.canada.ca/handle/123456789/2651
dc.language.iso: en
dc.publisher: Wiley
dc.relation.isreplacedby: https://doi.org/10.1002/em.22482
dc.rights.openaccesslevel - en: Green
dc.rights.openaccesslevel - fr: Vert
dc.subject - en: Nature and environment; Science and technology
dc.subject - fr: Nature et environnement; Sciences et technologie
dc.subject.en - en: Nature and environment; Science and technology
dc.subject.fr - fr: Nature et environnement; Sciences et technologie
dc.title - en: Microsatellite mutation frequencies in river otters (Lontra Canadensis) from the Athabasca Oil Sands region are correlated to polycyclic aromatic compound tissue burden
dc.type - en: Accepted manuscript
dc.type - fr: Manuscrit accepté
local.article.journalissue: 4
local.article.journaltitle: Environmental and Molecular Mutagenesis
local.article.journalvolume: 63
local.pagination: 31 pages
local.peerreview - en: Yes
local.peerreview - fr: Oui

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