Effect of muscle fibre types and carnosine levels on the expression of carnosine‑related genes in pig skeletal muscle

Calbe, Claudia; Metzger, Katharina; Gariépy, Claude; Palin, Marie-France

Effect of muscle fibre types and carnosine levels on the expression of carnosine‑related genes in pig skeletal muscle

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creativework.keywords - en: genes; gene expression; gènes; immunohistochemistry; muscle fibres; pigs; myoblasts
creativework.keywords - fr: expression génique; immunocytochimie; fibres musculaires; myoblastes; porcs
dc.contributor.author: Calbe, Claudia; Metzger, Katharina; Gariépy, Claude; Palin, Marie-France
dc.date.accepted: 2023-03-21
dc.date.accessioned: 2024-12-11T22:49:30Z
dc.date.available: 2024-12-11T22:49:30Z
dc.date.issued: 2023-05-12
dc.description.abstract - en: It is generally accepted that carnosine (β-alanyl-l-histidine) content is higher in glycolytic than in oxidative muscle fibres, but the underlying mechanisms responsible for this difference remain to be elucidated. A first study to better understand potential mechanisms involved was undertaken (1) to determine whether differences in the expression of carnosine-related enzymes (CARNS1, CNDP2) and transporters (SLC6A6, SLC15A3, SLC15A4, SLC36A1) exist between oxidative and glycolytic myofibres and (2) to study the effect of carnosine on myoblast proliferative growth and on carnosine-related gene expression in cultured myoblasts isolated from glycolytic and oxidative muscles. Immunohistochemistry analyses were conducted to determine the cellular localization of carnosine-related proteins. Laser-capture microdissection and qPCR analyses were performed to measure the expression of carnosine-related genes in different myofibres isolated from the longissimus dorsi muscle of ten crossbred pigs. Myogenic cells originating from glycolytic and oxidative muscles were cultured to assess the effect of carnosine (0, 10, 25 and 50 mM) on their proliferative growth and on carnosine-related gene expression. The mRNA abundance of CNDP2 and of the studied carnosine transporters was higher in oxidative than in glycolytic myofibres. Since carnosine synthase (CARNS1) mRNA abundance was not affected by either the fibre type or the addition of carnosine to myoblasts, its transcriptional regulation would not be the main process by which carnosine content differences are determined in oxidative and glycolytic muscles. The addition of carnosine to myoblasts leading to a dose-dependent increase in SLC15A3 transcripts, however, suggests a role for this transporter in carnosine uptake and/or efflux to maintain cellular homeostasis.
dc.identifier.citation: Kalbe, C., Metzger, K., Gariépy, C., & Palin, M-F. (2023). Effect of muscle fibre types and carnosine levels on the expression of carnosine-related genes in pig skeletal muscle. Histochemistry and Cell Biology, 160, 63–77. https://doi.org/10.1007/s00418-023-02193-6
dc.identifier.doi: https://doi.org/10.1007/s00418-023-02193-6
dc.identifier.issn: 1432-119X
dc.identifier.uri: https://open-science.canada.ca/handle/123456789/3204
dc.language.iso: en
dc.publisher - en: Springer
dc.rights - en: Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr: Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.uri - en: https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr: https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en: Livestock
dc.subject - fr: Bétail
dc.subject.en - en: Livestock
dc.subject.fr - fr: Bétail
dc.title - en: Effect of muscle fibre types and carnosine levels on the expression of carnosine‑related genes in pig skeletal muscle
dc.type - en: Article
dc.type - fr: Article
local.article.journaltitle - en: Histochemistry and Cell Biology
local.article.journalvolume: 160
local.pagination: 63-77
local.peerreview - en: Yes
local.peerreview - fr: Oui