MicroRNA roles in signalling during lactation : an insight from differential expression, time course and pathway analyses of deep sequence data

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DOI

https://doi.org/10.1038/srep44605

Language of the publication
English
Date
2017-03-20
Type
Article
Author(s)
  • Do, Duy N.
  • Li, Ran
  • Dudemaine, Pier-Luc
  • Ibeagha-Awemu, Eveline M.
Publisher
Nature Publishing Group

Abstract

The study examined microRNA (miRNA) expression and regulatory patterns during an entire bovine lactation cycle. Total RNA from milk fat samples collected at the lactogenesis (LAC, day1 [D1] and D7), galactopoiesis (GAL, D30, D70, D130, D170 and D230) and involution (INV, D290 and when milk production dropped to 5 kg/day) stages from 9 cows was used for miRNA sequencing. A total of 475 known and 238 novel miRNAs were identified. Fifteen abundantly expressed miRNAs across lactation stages play regulatory roles in basic metabolic, cellular and immunological functions. About 344, 366 and 209 miRNAs were significantly differentially expressed (DE) between GAL and LAC, INV and GAL, and INV and LAC stages, respectively. MiR-29b/miR-363 and miR-874/miR-6254 are important mediators for transition signals from LAC to GAL and from GAL to INV, respectively. Moreover, 58 miRNAs were dynamically DE in all lactation stages and 19 miRNAs were significantly time-dependently DE throughout lactation. Relevant signalling pathways for transition between lactation stages are involved in apoptosis (PTEN and SAPK/JNK), intracellular signalling (protein kinase A, TGF-β and ERK5), cell cycle regulation (STAT3), cytokines, hormones and growth factors (prolactin, growth hormone and glucocorticoid receptor). Overall, our data suggest diverse, temporal and physiological signal-dependent regulatory and mediator functions for miRNAs during lactation.

Subject

  • Cattle,
  • Animal research

Keywords

  • Gene expression,
  • High-Throughput Nucleotide Sequencing,
  • Lactation,
  • MicroRNA,
  • Cattle,
  • Polymerase chain reaction,
  • Cellular signal transduction

Rights

Pagination

1-19

Peer review

Yes

Identifiers

ISSN
2045-2322

Article

Journal title
Scientific Reports
Journal volume
7
Article number
44605
Accepted date
2017-02-09
Submitted date
2016-10-05

Citation(s)

Do, D. N., Li, R., Dudemaine, P.-L., & Ibeagha-Awemu, E. M. (2017). MicroRNA roles in signalling during lactation : an insight from differential expression, time course and pathway analyses of deep sequence data. Scientific Reports, 7, Article 44605. https://doi.org/10.1038/srep44605

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Animals and insects

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