Long chain fatty acids analysis of intertidal biofilm by direct injection liquid chromatography time of flight mass spectrometry

Simple item page

Simple item page

Full item details

dc.contributor.author
Brunswick, Pamela
Blajkevitch, Oxana
Filewood, Taylor
Kent, Emma
Drever, Mark C.
Elner, Robert W.
Shang, Dayue
dc.date.accepted
2023-02-10
dc.date.accessioned
2025-04-03T19:21:59Z
dc.date.available
2025-04-03T19:21:59Z
dc.date.issued
2023-03-29
dc.date.submitted
2023-01-05
dc.description.abstract - en
The critical importance of mono- and polyunsaturated fatty acids (FAs) in a variety of biological functions, including animal nutrition and as an environmental stress monitor, is well recognized. However, while methods exist for monitoring of fatty acids, few are specific either to the profile of a microphytobenthos matrix or practical in application to multiple, diverse intertidal biofilm sample sets. In the current study, a sensitive liquid chromatography (LC) quadrupole time of flight mass spectrometry (QTOF) method was developed for the quantitative analysis of 31 FAs specific to intertidal biofilm, a thin mucilaginous layer of microalgae, bacteria, and other organisms on the surface of coastal mudflats, which provide a rich source of FAs for migratory birds. Preliminary screening of diverse biofilm samples collected from shorebird feeding grounds highlighted eight saturated (SFA), seven monounsaturated (MUFA), and sixteen polyunsaturated FAs (PUFA) that were selected for analysis. Improved method detection limits in the range 0.3 ngmL<sup>-1</sup> to 2.6 ngmL<sup>-1</sup> were achieved, excepting for stearic acid at 10.6 ngmL<sup>-1</sup>. These excellent results were obtained without use of complex sample extraction and clean-up procedures undertaken by other published methods. An alkaline matrix of dilute aqueous ammonium hydroxide with methanol was shown to be selective for extraction and stability of the more hydrophilic fatty acid components. The direct injection method showed excellent precision and accuracy both during validation and application to hundreds of real-world intertidal biofilm samples from the Fraser River estuary (British Columbia, Canada) and other areas of the region frequented by shoreline birds.
dc.identifier.issn
1873-3778
0021-9673
dc.identifier.uri
https://open-science.canada.ca/handle/123456789/3555
dc.language.iso
en
dc.publisher - en
Elsevier
dc.relation.isreplacedby
https://doi.org/10.1016/j.chroma.2023.463870
dc.rights - en
Open Government Licence - Canada
dc.rights - fr
Licence du gouvernement ouvert - Canada
dc.rights.openaccesslevel - en
Green
dc.rights.openaccesslevel - fr
Vert
dc.rights.uri - en
https://open.canada.ca/en/open-government-licence-canada
dc.rights.uri - fr
https://ouvert.canada.ca/fr/licence-du-gouvernement-ouvert-canada
dc.subject - en
Migratory birds
Estuaries
dc.subject - fr
Oiseau migrateur
Estuaire
dc.subject.en - en
Migratory birds
Estuaries
dc.subject.fr - fr
Oiseau migrateur
Estuaire
dc.title - en
Long chain fatty acids analysis of intertidal biofilm by direct injection liquid chromatography time of flight mass spectrometry
dc.type - en
Submitted manuscript
dc.type - fr
Manuscrit soumis
local.acceptedmanuscript.articlenum
463870
local.article.journaltitle - en
Journal of Chromatography A
local.article.journalvolume
1693
local.pagination
99 pages
local.peerreview - en
No
local.peerreview - fr
Non
Download(s)

Original bundle

Now showing 1 - 1 of 1

Thumbnail image

Name: LongChainFattyAcidsAnalysisIntertidalBiofilmDirectInjectionLiquidChromatographyTimeFlightMassSpectrometry.pdf

Size: 3.83 MB

Format: PDF

Download file

Collection(s)

Page details

Date modified: