The Complete Genome Sequence of Bacillus thuringiensis Strain ATCC 13367 Features a cry-Containing Chromosome
- DOI
- Language of the publication
- English
- Date
- 2022-04-04
- Type
- Article
- Author(s)
- Shwed, P. S.
- Crosthwait, J.
- Weedmark, K.
- Leveque, G.
- Publisher
- American Society for Microbiology
Abstract
We report the high-quality closed genome sequence of Bacillus thuringiensis ATCC 13367, which is used as a bioinsecticide. The genome features a 328-kbp plasmid and a 5.8-Mbp chromosome. The genome is atypical for the species since it lacks a plasmid pesticidal crystal protein (cry) gene and instead features a chromosomal cry gene like that of B. thuringiensis HER1410.
Plain language summary
Health Canada assesses and manages risks of biotechnology microorganisms under the Canadian Environmental Protection Act (CEPA) including microorganisms on the Domestic Substance List (DSL). Bacillus thuringiensis (Bt) ATCC 13367 is a microorganism used as a biopesticide and part of the Domestic Substance List (DSL ). As part of an earlier screening assessment, It was previously whole genome sequenced as multiple pieces. For this report, in order to generate a complete sequence, more recent sequencing technologies and bioinformatics tools were employed. The complete genome shows the presence of genes implicated in food poisoning along with an insecticidal protein gene located in an unusual location. The new observations contribute to an improved understanding of this microorganism and the methodologies described in this report will influence future biotechnology microorganism genome analyses.
Subject
- Health,
- Health and safety