An efficient viral vector for functional genomic studies of Prunus fruit trees and its induced resistance to Plum pox virus via silencing of a host factor gene
An efficient viral vector for functional genomic studies of Prunus fruit trees and its induced resistance to Plum pox virus via silencing of a host factor gene
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Full item details
- creativework.keywords - en
- RNA
- Plant gene silencing
- Prunus--Viruses
- Fruit trees--Diseases and pest resistance
- creativework.keywords - fr
- ARN
- Plantes--Inactivation génique
- Prunus--Virus
- Arbres fruitiers--Résistance aux maladies et fléaux
- dc.contributor.author
- Cui, Hongguang
- Wang, Aiming
- dc.date.accepted
- 2016-08-27
- dc.date.accessioned
- 2025-10-06T17:44:34Z
- dc.date.available
- 2025-10-06T17:44:34Z
- dc.date.issued
- 2016-08-27
- dc.date.submitted
- 2016-06-08
- dc.description.abstract - en
- RNA silencing is a powerful technology for molecular characterization of gene functions in plants. A commonly used approach to the induction of RNA silencing is through genetic transformation. A potent alternative is to use a modified viral vector for virus-induced gene silencing (VIGS) to degrade RNA molecules sharing similar nucleotide sequence. Unfortunately, genomic studies in many allogamous woody perennials such as peach are severely hindered because they have a long juvenile period and are recalcitrant to genetic transformation. Here, we report the development of a viral vector derived from Prunus necrotic ringspot virus (PNRSV), a widespread fruit tree virus that is endemic in all Prunus fruit production countries and regions in the world. We show that the modified PNRSV vector, harbouring the sense-orientated target gene sequence of 100-200 bp in length in genomic RNA3, could efficiently trigger the silencing of a transgene or an endogenous gene in the model plant Nicotiana benthamiana. We further demonstrate that the PNRSV-based vector could be manipulated to silence endogenous genes in peach such as eukaryotic translation initiation factor 4E isoform (eIF(iso)4E), a host factor of many potyviruses including Plum pox virus (PPV). Moreover, the eIF(iso)4E-knocked down peach plants were resistant to PPV. This work opens a potential avenue for the control of virus diseases in perennial trees via viral vector-mediated silencing of host factors, and the PNRSV vector may serve as a powerful molecular tool for functional genomic studies of Prunus fruit trees.
- dc.identifier.citation
- Cui, H., & Wang, A. (2017). An efficient viral vector for functional genomic studies of Prunus fruit trees and its induced resistance to Plum pox virus via silencing of a host factor gene. Plant Biotechnology Journal, 15(3), 344-356. https://doi.org/10.1111/pbi.12629
- dc.identifier.doi
- https://doi.org/10.1111/pbi.12629
- dc.identifier.issn
- 1467-7652
- 1467-7644
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3909
- dc.language.iso
- en
- dc.publisher - en
- Blackwell Publishing Ltd
- dc.publisher - fr
- Blackwell Publishing Ltd
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Genetics
- Plant diseases
- Fruit crops
- dc.subject - fr
- Génétique
- Maladie des plantes
- Cultures fruitières
- dc.subject.en - en
- Genetics
- Plant diseases
- Fruit crops
- dc.subject.fr - fr
- Génétique
- Maladie des plantes
- Cultures fruitières
- dc.title - en
- An efficient viral vector for functional genomic studies of Prunus fruit trees and its induced resistance to Plum pox virus via silencing of a host factor gene
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journalissue
- 3
- local.article.journaltitle - en
- Plant Biotechnology Journal
- local.article.journalvolume
- 15
- local.pagination
- 344-356
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
- local.requestdoi - en
- No
- local.requestdoi - fr
- No
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