Impact of Sinorhizobium meliloti strains and plant population on regrowth and nodule regeneration of alfalfa after a freezing event

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creativework.keywords - en

alfalfa

freezing stress

nodules

cold adaptation

symbiosis

acclimatization

creativework.keywords - fr

luzerne

symbiose

nodosités

stress dû au gel

adaptation au froid

acclimatation

dc.contributor.author

D'Amour, Emmanuelle

Bertrand, Annick

Cloutier, Jean

Claessens, Annie

Rocher, Solen

Seguin, Philippe

dc.date.accepted

2022-08-14

dc.date.accessioned

2024-11-04T21:06:55Z

dc.date.available

2024-11-04T21:06:55Z

dc.date.issued

2022-08-25

dc.date.submitted

2022-06-30

dc.description.abstract - en

Purpose The increase in frequency of freeze–thaw episodes with the diminution of snow cover protection due to climate change compromises the winter survival of alfalfa (Medicago sativa L.). Symbiosis with cold-tolerant rhizobial strains can improve the ability of alfalfa to survive and grow under stressful conditions. Methods Six strains of Sinorhizobium (Ensifer) meliloti were tested in combination with two alfalfa populations bred to differ in their levels of freezing tolerance. Plants of each different combination were grown for eight weeks in a growth chamber before being exposed to temperatures promoting their acclimation to cold. Plants were then exposed to a freezing stress (-11ºC) and regrown for three weeks. Shoot, root and nodule biomass were measured before cold acclimation and three weeks after the freezing stress. Results After freezing stress, the alfalfa population A-TF7 had shoot and root biomasses that were respectively 19% and 15% larger than cultivar A-TF0. Alfalfa plants inoculated with strain NRG34 showed both a larger shoot biomass and a higher nodule dry weight than plants inoculated with any other strains. Assessment of freezing damages on nodules showed that plants inoculated with NRG34 had the largest proportion of undamaged nodules or of nodules with a regeneration zone. Conclusion This study shows for the first time a relationship between nodule and shoot regrowth after a freezing stress, the latter being linked with the proportion of nodules showing less freezing damage. Our results demonstrated that both the choice of alfalfa populations and S. meliloti strains adapted to stress are complementary to increasing alfalfa persistence.

dc.identifier.citation

D’Amours, E., Bertrand, A., Cloutier, J., Claessens, A., Rocher, S., & Seguin, P. (2022). Impact of Sinorhizobium meliloti strains and plant population on regrowth and nodule regeneration of alfalfa after a freezing event. Plant and Soil, 500(1-2), 161–179. https://doi.org/10.1007/s11104-022-05662-4

dc.identifier.doi

https://doi.org/10.1007/s11104-022-05662-4

dc.identifier.issn

1573-5036

0032-079X

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/3105

dc.language.iso

en

dc.publisher - en

Springer Nature

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Crops

Agriculture

dc.subject - fr

Cultures

Agriculture

dc.subject.en - en

Crops

Agriculture

dc.subject.fr - fr

Cultures

Agriculture

dc.title - en

Impact of Sinorhizobium meliloti strains and plant population on regrowth and nodule regeneration of alfalfa after a freezing event

dc.type - en

Article

dc.type - fr

Article

local.article.journalissue

1-2

local.article.journaltitle - en

Plant and Soil

local.article.journalvolume

500

local.pagination

161-179

local.peerreview - en

Yes

local.peerreview - fr

Oui

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