Histopathology of Sclerotinia sclerotiorum infection and oxalic acid function in susceptible and resistant soybean

Simple item page

Simple item page

Full item details

creativework.keywords - en

Sclerotinia sclerotiorum

Sclerotinia sclerotiorum

Champignons phytopathogènes

Phytopathogenic fungi

creativework.keywords - fr

Acide oxalique

Oxalic acid

Soja--Maladies et fléaux

Soybean--Diseases and pests

dc.contributor.author

Davidson, A. L.

Blahut-Beatty, L.

Itaya, A.

Zhang, Y.

Zheng, S.

Simmonds, D.

dc.date.accessioned

2024-10-21T15:29:44Z

dc.date.available

2024-10-21T15:29:44Z

dc.date.issued

2016-03-31

dc.description.abstract - en

The success of the necrotrophic fungus Sclerotinia sclerotiorum is largely dependent on its major virulence factor, oxalic acid (OA). Virulence is lost in transgenic plants that express OA degrading enzymes, e.g. oxalate oxidase (OxO). The histopathology of S. sclerotiorum infection and OA accumulation was examined in a transgenic soybean line over-expressing OxO (OxO-OE) and its isogenic parent (WT). In situ flower inoculation showed that the OxO-OE plants were highly resistant to the pathogen while the WT parents were susceptible. This difference in resistance was not apparent in the floral tissues, as aggressive hyphal activity was similar on both hosts, showing that high OxO activity and low OA accumulation in OxO-OE was not a deterrent. However, the process of fungal infection on excised leaf tissue differed on the two hosts. Primary lesions developed and showed similar severe ultrastructural damage on both hosts but rapid lesion expansion (colonization) proceeded only on the WT, concomitant with OA accumulation. Oxalic acid rose in OxO-OE 1 day post-inoculation and did not change over the following 3 days, showing that colonization can be blocked by maintaining low levels of OA. However, OxO degradation of OA did not deter initial host penetration and primary lesion formation. This shows that OA, the major virulence factor of S. sclerotiorum, is critical for host colonization but may not be required during primary lesion formation, suggesting that other factors are contributing to the establishment of the primary lesion.

dc.identifier.citation

Davidson, A. L., Blahut-Beatty, L., Itaya, A., Zhang, Y., Zheng, S., & Simmonds, D. (2016). Histopathology of Sclerotinia sclerotiorum infection and oxalic acid function in susceptible and resistant soybean. Plant Pathology, 65(6), 878-887. https://doi.org/10.1111/ppa.12514

dc.identifier.doi

https://doi.org/10.1111/ppa.12514

dc.identifier.issn

0032-0862

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/3079

dc.language.iso

en

dc.publisher - en

Blackwell Publishing Ltd

dc.publisher - fr

Blackwell Publishing Ltd

dc.rights - en

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

dc.rights - fr

Creative Commons Attribution - Pas d'utilisation commerciale - Pas de modification 4.0 International (CC BY-NC-ND 4.0)

dc.rights.openaccesslevel - en

Gold

dc.rights.openaccesslevel - fr

Or

dc.rights.uri - en

https://creativecommons.org/licenses/by-nc-nd/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by-nc-nd/4.0/deed.fr

dc.subject - en

Agriculture

dc.subject - fr

Agriculture

dc.subject.en - en

Agriculture

dc.subject.fr - fr

Agriculture

dc.title - en

Histopathology of Sclerotinia sclerotiorum infection and oxalic acid function in susceptible and resistant soybean

dc.type - en

Article

dc.type - fr

Article

local.article.journalissue

6

local.article.journaltitle

Plant Pathology

local.article.journalvolume

65

local.pagination

878-887

local.peerreview - en

Yes

local.peerreview - fr

Oui

local.requestdoi - en

No

local.requestdoi - fr

No

Download(s)

Original bundle

Now showing 1 - 1 of 1

Name: HistopathologyOfSclerotiniaSclerotiorumInfectionAndOxalicAcidFunctionInSusceptibleAndResistantSoybean_2016.pdf

Size: 1.21 MB

Format: PDF

Download file