Physiological and biochemical responses to salt stress of alfalfa populations selected for salinity tolerance and grown in symbiosis with salt-tolerant rhizobium

Bertrand, A.; Gatzke, C.; Bipfubusa, M.; Lévesque, V.; Chalifour, F. C.; Claessens, A.; Rocher, S.; Tremblay, G. F.; Beauchamp, C. J.

doi:https://open-science.canada.ca/handle/123456789/235

Physiological and biochemical responses to salt stress of alfalfa populations selected for salinity tolerance and grown in symbiosis with salt-tolerant rhizobium

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creativework.keywords - en: salinity tolerance; rhizobium; nodules; amino acids; sucrose
creativework.keywords - fr: tolérance à la salinité; rhizobium; nodosités; acides aminés; saccharose
dc.contributor.author: Bertrand, A.; Gatzke, C.; Bipfubusa, M.; Lévesque, V.; Chalifour, F. C.; Claessens, A.; Rocher, S.; Tremblay, G. F.; Beauchamp, C. J.
dc.date.accessioned: 2023-04-20T20:34:36Z
dc.date.available: 2023-04-20T20:34:36Z
dc.date.issued: 2020-04-15
dc.description - EN: Salinity is an important abiotic stress and a major threat to crop productivity worldwide In Canada,approximately 30% (20 million ha) of agricultural land either openly shows salinization or is at risk ofbecoming salinized In addition, soil salinization problems will likely worsen in the context ofclimate change, owing to the predicted increases of temperature and changes in precipitation patternsworldwide and to the increasing use of poor-quality water for irrigation worlwide Alfalfa, the most important legume forage crop in Canda, is sensitive to salt stress In an effort to increase the salinity resistance of alfalfa, we proceeded with the selection of salt-resistant alfalfa populations at the Salt Lab in Swift Current (SK) Here we tested under salinity stress two alfalfa populations obtained after three cycles of selection, in association with a salt resistant rhizobia strain to ascertain the improvement of salinity resistance in response to selection Our results shows that our selection method is very efficient at improving salt tolerance since the selected alfalfa populations had significantly higher yields than initial populations under salt stress When looking at the mechanisms of resistance, we found that the osmotic adjustment both in the plant (leaves and roots) and at in the nodules containing rhizobium were key to the increased salt resistance and higher yield
dc.description.abstract - en: Alfalfa and its rhizobial symbiont are sensitive to salinity. We compared the physiological responses of alfalfa populations inoculated with a salt-tolerant rhizobium strain, exposed to five NaCl concentrations (0, 20, 40, 80, or 160 mM NaCl). Two initial cultivars, Halo (H-TS0) and Bridgeview (B-TS0), and two populations obtained after three cycles of recurrent selection for salt tolerance (H-TS3 and B-TS3) were compared. Biomass, relative water content, carbohydrates, and amino acids concentrations in leaves and nodules were measured. The higher yield of TS3-populations than initial cultivars under salt stress showed the effectiveness of our selection method to improve salinity tolerance. Higher relative root water content in TS3 populations suggests that root osmotic adjustment is one of the mechanisms of salt tolerance. Higher concentrations of sucrose, pinitol, and amino acid in leaves and nodules under salt stress contributed to the osmotic adjustment in alfalfa. Cultivars differed in their response to recurrent selection: under a 160 mM NaCl-stress, aromatic amino acids and branched-chain amino acids (BCAAs) increased in nodules of B-ST3 as compared with B-TS0, while these accumulations were not observed in H-TS3. BCAAs are known to control bacteroid development and their accumulation under severe stress could have contributed to the high nodulation of B-TS3.
dc.description.fosrctranslation - FR: La salinité est un stress abiotique important et une menace majeure pour la productivité des cultures dans le monde entier Au Canada, environ 30 % (20 millions d’hectares) des terres agricoles sont exposées à la salinisation ou risquent de le devenir De plus, les problèmes de salinisation des sols s’aggraveront probablement dans le contexte du changement climatique, en raison des augmentations prévues de la température et des changements dans les régimes de précipitations dans le monde entier et de l’utilisation croissante d’eau de mauvaise qualité pour l’irrigation à l'échelle internationale La luzerne, la légumineuse fourragère la plus importante au Canada, est sensible au stress salin Dans le but d’accroître la résistance de la luzerne à la salinité, nous avons procédé à la sélection de populations de luzerne résistantes au sel au Salt Lab de Swift Current (SK) Dans le cadre de la présente étude, nous avons soumis à un stress salin deux populations de luzerne obtenues après trois cycles de sélection, en association avec une souche de rhizobiums résistante au sel, afin de vérifier l’amélioration de la résistance à la salinité en réponse à la sélection Nos résultats montrent que notre méthode de sélection est très efficace pour améliorer la tolérance au sel, car les populations de luzerne sélectionnées ont donné des rendements significativement plus élevés que les populations initiales soumises à un stress salin En examinant les mécanismes de la résistance, nous avons constaté que l’ajustement osmotique de la plante (feuilles et racines) et des nœuds contenant du rhizobium était la clé de l’augmentation de la résistance au sel et de l’augmentation du rendement
dc.identifier.citation: Bertrand, A., Gatzke, C., Bipfubusa, M., Lévesaque, V., Chalifour, F. P., Claessens, A., Rocher, S., Tremblay, G. F., & Beauchamp, C. J. (2020). Physiological and biochemical responses to salt stress of alfalfa populations selected for salinity tolerance and grown in symbiosis with salt-tolerant rhizobium. Agronomy, 10(4). https://doi.org/10.3390/agronomy10040569
dc.identifier.doi: https://doi.org/10.3390/agronomy10040569
dc.identifier.issn: 2073-4395
dc.identifier.uri: https://open-science.canada.ca/handle/123456789/235
dc.language.iso: en
dc.publisher: MDPI
dc.rights.openaccesslevel - en: Gold
dc.rights.openaccesslevel - fr: Or
dc.subject - en: Agriculture
dc.subject - fr: Agriculture
dc.subject.en - en: Agriculture
dc.subject.fr - fr: Agriculture
dc.title - en: Physiological and biochemical responses to salt stress of alfalfa populations selected for salinity tolerance and grown in symbiosis with salt-tolerant rhizobium
dc.title.fosrctranslation - fr: Physiological and biochemical responses to salt stress of alfalfa populations selected for salinity tolerance and grown in symbiosis with salt-tolerant rhizobium
dc.type - en: Article
dc.type - fr: Article
local.article.journalissue: 4
local.article.journaltitle: Agronomy
local.article.journalvolume: 10
local.peerreview - en: Yes
local.peerreview - fr: Oui

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