Impact of a primary wastewater effluent on liver lipid metabolism and oxidative stress in St. Lawrence River Northern pike

Meunier, Mélanie; Hanana, Houda; Houde, Magali; Rosabal, Maikel; Sauvé, Sébastien; Verreault, Jonathan

doi:https://open-science.canada.ca/handle/123456789/3636

Impact of a primary wastewater effluent on liver lipid metabolism and oxidative stress in St. Lawrence River Northern pike

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dc.contributor.author: Meunier, Mélanie; Hanana, Houda; Houde, Magali; Rosabal, Maikel; Sauvé, Sébastien; Verreault, Jonathan
dc.date.accepted: 2025-04-03
dc.date.accessioned: 2025-05-29T15:52:09Z
dc.date.available: 2025-05-29T15:52:09Z
dc.date.issued: 2025-05-25
dc.date.submitted: 2024-09-25
dc.description.abstract - en: Municipal wastewater effluents (MWWEs) contain complex chemical mixtures that can affect the health of exposed aquatic organisms. Montreal’s (Quebec, Canada) primary MWWE is one of the largest in North America and is a known point of release of halogenated flame retardants (HFRs) and per- and polyfluoroalkyl substances (PFAS) into the St. Lawrence River ecosystem. The objective of this study was to investigate hepatic lipid metabolism and oxidative stress in St. Lawrence River Northern pike (Esox lucius) environmentally exposed to Montreal's MWWE. A suite of HFRs and PFAS were also measured in pike liver. Among the 76 PFAS that were investigated in pike, 18 compounds were analyzed for the first time in St. Lawrence River fish, although only three of these could be detected and quantified. Concentrations of ∑76PFAS in liver of female pike collected downstream of the effluent outfall were 32% lower than those collected upstream. In male pike liver, 0.3-fold lower mRNA levels of peroxisome proliferator-activated receptor alpha (pparα), a regulator of lipid metabolism, and 0.7-fold lower levels of phospholipase A2 mRNA (pla2g4ab), involved in lysophosphatidylcholine and arachidonic acid metabolism, were observed. Additionally, there was a 17% decrease in relative abundance of the ∑1528lipid at the downstream site for males. Higher percentages of contribution to the ∑1528lipid were noted for ∑1103membrane lipids (26% higher) and ∑2steroid lipids (50% higher) in male pike collected at the downstream site. Moreover, negative correlations between ∑34PBDE concentrations and pparα mRNA levels as well as between ∑2steroid lipids and pla2g4ab mRNA levels were determined in male pike. These findings suggest that chronic environmental exposure of a top predator fish to a primary MWWE may have sex-specific effects on liver lipid content and composition as well as the transcription of genes involved in lipid metabolism.
dc.description.plainlanguage - en: The objective of this study was to investigate lipid metabolism and oxidative stress in liver of St. Lawrence River northern pike (Esox lucius) environmentally exposed to Montreal’s municipal wastewater effluent. Perfluoroalkyl substances and flame retardants were quantified in fish. Findings suggest that chronic environmental exposure of a top predator fish to a primary urban effluent may have sex-specific effects on liver lipid composition and the transcription of genes involved in lipid metabolism.
dc.description.plainlanguage - fr: L’objectif de cette étude était d’étudier le métabolisme des lipides et le stress oxydatif dans le foie du grand brochet (Esox lucius) du fleuve Saint-Laurent exposé à l’effluent des eaux usées municipales de Montréal. Les substances perfluoroalkylées et les produits ignifuges ont été quantifiés dans les poissons. Les résultats suggèrent que l’exposition environnementale chronique d’un poisson prédateur de niveau trophique supérieur à un effluent urbain primaire peut avoir des effets spécifiques au sexe sur la composition des lipides du foie et la transcription des gènes impliqués dans le métabolisme des lipides.
dc.identifier.issn: 1879-1026; 0048-9697
dc.identifier.uri: https://open-science.canada.ca/handle/123456789/3636
dc.language.iso: en
dc.publisher - en: Elsevier
dc.relation.isreplacedby: https://doi.org/10.1016/j.scitotenv.2025.179349
dc.rights - en: Open Government Licence - Canada
dc.rights - fr: Licence du gouvernement ouvert - Canada
dc.rights.openaccesslevel - en: Green
dc.rights.openaccesslevel - fr: Vert
dc.rights.uri - en: https://open.canada.ca/en/open-government-licence-canada
dc.rights.uri - fr: https://ouvert.canada.ca/fr/licence-du-gouvernement-ouvert-canada
dc.subject - en: Waste water; Freshwater fish; Rivers; Water pollution; Water quality
dc.subject - fr: Eaux usées; Poisson d'eau douce; Fleuve / Rivière; Pollution de l'eau; Qualité de l'eau
dc.subject.en - en: Waste water; Freshwater fish; Rivers; Water pollution; Water quality
dc.subject.fr - fr: Eaux usées; Poisson d'eau douce; Fleuve / Rivière; Pollution de l'eau; Qualité de l'eau
dc.title - en: Impact of a primary wastewater effluent on liver lipid metabolism and oxidative stress in St. Lawrence River Northern pike
dc.title.alternative: Incidence d’un effluent d’eau usée primaire sur le métabolisme des lipides du foie et le stress oxydatif chez le grand brochet du fleuve Saint-Laurent
dc.type - en: Submitted manuscript
dc.type - fr: Manuscrit soumis
local.acceptedmanuscript.articlenum: 179349
local.article.journaltitle - en: Science of The Total Environment
local.article.journalvolume: 978
local.pagination: 44 pages
local.peerreview - en: No
local.peerreview - fr: Non

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