Identification of new leaf rust resistance Loci in wheat and wild relatives by array-based SNP genotyping and association genetics

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dc.contributor.author

Fatima, Fizza

McCallum, Brent D.

Pozniak, Curtis J.

Hiebert, Colin W.

McCartney, Curt A.

Fedak, George

You, Frank M.

Cloutier, Sylvie

dc.date.accepted

2020-10-15

dc.date.accessioned

2025-02-04T21:48:45Z

dc.date.available

2025-02-04T21:48:45Z

dc.date.issued

2020-11-15

dc.date.submitted

2020-07-19

dc.description.abstract - en

Leaf rust caused by Puccinia triticina is the most widespread rust disease of wheat. As pathogen populations are constantly evolving, identification of novel sources of resistance is necessary to maintain disease resistance and stay ahead of this plant-pathogen evolutionary arms race. The wild genepool of wheat is a rich source of genetic diversity, accounting for 44% of the Lr genes identified. Here we performed a genome-wide association study (GWAS) on a diverse germplasm of 385 accessions, including 27 different Triticum and Aegilops species. Genetic characterization using the wheat 90 K array and subsequent filtering identified a set of 20,501 single nucleotide polymorphic (SNP) markers. Of those, 9,570 were validated using exome capture and mapped onto the Chinese Spring reference sequence v1.0. Phylogenetic analyses illustrated four major clades, clearly separating the wild species from the T. aestivum and T. turgidum species. GWAS was conducted using eight statistical models for infection types against six leaf rust isolates and leaf rust severity rated in field trials for 3–4 years at 2–3 locations in Canada. Functional annotation of genes containing significant quantitative trait nucleotides (QTNs) identified 96 disease-related loci associated with leaf rust resistance. A total of 21 QTNs were in haplotype blocks or within flanking markers of at least 16 known Lr genes. The remaining significant QTNs were considered loci that putatively harbor new Lr resistance genes. Isolation of these candidate genes will contribute to the elucidation of their role in leaf rust resistance and promote their usefulness in marker-assisted selection and introgression.

dc.identifier.citation

Fatima, F., McCallum, B. D., Pozniak, C. J., Hiebert, C. W., McCartney, C. A., Fedak, G., You, F. M., & Cloutier, S. (2020). Identification of new leaf rust resistance loci in wheat and wild relatives by array-based SNP genotyping and association genetics. Frontiers in Plant Science, 11. https://doi.org/10.3389/fpls.2020.583738

dc.identifier.doi

https://doi.org/10.3389/fpls.2020.583738

dc.identifier.issn

1664-462X

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/3394

dc.language.iso

en

dc.publisher - en

Frontiers Media S.A.

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Agriculture

Plant diseases

Wheat

Genetics

dc.subject - fr

Agriculture

Maladie des plantes

Blé

Génétique

dc.subject.en - en

Agriculture

Plant diseases

Wheat

Genetics

dc.subject.fr - fr

Agriculture

Maladie des plantes

Blé

Génétique

dc.title - en

Identification of new leaf rust resistance Loci in wheat and wild relatives by array-based SNP genotyping and association genetics

dc.type - en

Article

dc.type - fr

Article

local.acceptedmanuscript.articlenum

583738

local.article.journaltitle - en

Frontiers in Plant Science

local.article.journalvolume

11

local.peerreview - en

Yes

local.peerreview - fr

Oui

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