Hiding in plain sight: discovery and phylogeography of a cryptic species of Trichinella (Nematoda: Trichinellidae) in wolverine (Gulo gulo)

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creativework.keywords - en
Trichinellosis and other foodborne zoonotic pathogens
Trichinella
creativework.keywords - fr
Trichinellose et autres pathogènes zoonotiques d'origine alimentaire
Trichinella
dc.contributor.author
Sharma, Rajnish
Thompson, Peter C.
Hoberg, Eric P.
Scandrett, Brad W.
Konecsni, Kelly
Harms, N. Jane
Kukka, Piia M.
Jung, Thomas S.
Elkin, Brett
Mulders, Robert
Larter, Nicholas C.
Branigan, Marsha
Pongracz, Jodie
Wagner, Brent
Kafle, Pratap
Lobanov, Vladislav A.
Rosenthal, Benjamin M.
Jenkins, Emily J.
dc.date.accepted
2020-01-16
dc.date.accessioned
2025-03-14T18:06:42Z
dc.date.available
2025-03-14T18:06:42Z
dc.date.issued
2020-03-24
dc.date.submitted
2019-07-31
dc.description.abstract - en
Understanding parasite diversity and distribution is essential in managing the potential impact of parasitic diseases in animals and people. Imperfect diagnostic methods, however, may conceal cryptic species. Here, we report the discovery and phylogeography of a previously unrecognized species of Trichinella in wolverine (Gulo gulo) from northwestern Canada that was indistinguishable from T. nativa using the standard multiplex PCR assay based on the expansion segment 5 (ESV) of ribosomal DNA. The novel genotype, designated as T13, was discovered when sequencing the mitochondrial genome. Phylogenetic analyses of the mitochondrial genome and of 15 concatenated single copy orthologs of nuclear DNA indicated a common ancestor for the encapsulated clade is shared by a subclade containing Trichinella spiralis and Trichinella nelsoni, and a subclade containing T13 and remaining taxa: T12 + (T2 + T6) + [(T5 + T9) + (T3 + T8)]. Of 95 individual hosts from 12 species of mammalian carnivores from northwestern Canada from which larvae were identified as T. nativa on multiplex PCR, only wolverines were infected with T13 (14 of 42 individuals). These infections were single or mixed with T. nativa and/or T6. Visual examination and motility testing confirmed that T13 is encapsulated and likely freeze-tolerant. We developed a new Polymerase Chain Reaction-Restriction Fragment Length Polymorphism which unequivocally distinguishes between T13 and T. nativa. We propose Trichinella chanchalensis n. sp. for T13, based on significant genetic divergence from other species of Trichinella and broad-based sampling of the Trichinella genome. Exploration of Alaskan and Siberian isolates may contribute to further resolution of a phylogeographically complex history for species of Trichinella across Beringia, including Trichinella chanchalensis n. sp. (T13).
dc.identifier.citation
Sharma, R., Thompson, P. C., Hoberg, E. P., Brad , B. W., Konecsni, K., Harms, N. J., Kukka, P. M., Jung, T. S., Elkin, B., Mulders, R., Larter, N. C., Branigan, M., Pongracz, J., Wagner, B., Kafle, P., Lobanov, V. A., Rosenthal, B. M., & Jenkins, E. J. (2020). Hiding in plain sight: Discovery and phylogeography of a cryptic species of trichinella (Nematoda: Trichinellidae) in Wolverine (gulo gulo). International Journal for Parasitology, 50(4), 277–287. https://doi.org/10.1016/j.ijpara.2020.01.003
dc.identifier.uri
https://open-science.canada.ca/handle/123456789/3522
dc.language.iso
en
dc.publisher - en
Elsevier
dc.rights - en
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.uri - en
https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr
https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en
Animal diseases
dc.subject - fr
Maladie animale
dc.subject.en - en
Animal diseases
dc.subject.fr - fr
Maladie animale
dc.title - en
Hiding in plain sight: discovery and phylogeography of a cryptic species of Trichinella (Nematoda: Trichinellidae) in wolverine (Gulo gulo)
dc.type - en
Article
dc.type - fr
Article
local.article.journalissue
4
local.article.journaltitle - en
International Journal for Parasitology
local.article.journalvolume
50
local.pagination
277-287
local.peerreview - en
Yes
local.peerreview - fr
Oui
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