Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum
- DOI
- Language of the publication
- English
- Date
- 2020-03-03
- Type
- Article
- Author(s)
- Zaheer, Rahat
- Cook, Shaun R.
- Barbieri, Ruth
- Goji, Noriko
- Cameron, Andrew
- Petkau, Aaron
- Ortega Polo, Rodrigo
- Tymensen, Lisa
- Stamm, Courtney
- Song, Jiming
- Hannon, Sherry
- Jones, Tineke
- Church, Deirdre
- Booker, Calvin W.
- Amoako, Kingsley
- Van Domselaar, Gary
- Read, Ron R.
- McAllister, Tim A.
- Publisher
- Springer Nature
Abstract
For a One-Health investigation of antimicrobial resistance (AMR) in Enterococcus spp., isolates from humans and beef cattle along with abattoirs, manured fields, natural streams, and wastewater from both urban and cattle feedlot sources were collected over two years. Species identification of Enterococcus revealed distinct associations across the continuum. Of the 8430 isolates collected, Enterococcus faecium and Enterococcus faecalis were the main species in urban wastewater (90%) and clinical human isolates (99%); Enterococcus hirae predominated in cattle (92%) and feedlot catch-basins (60%), whereas natural streams harbored environmental Enterococcus spp. Whole-genome sequencing of E. faecalis (n = 366 isolates) and E. faecium (n = 342 isolates), revealed source clustering of isolates, indicative of distinct adaptation to their respective environments. Phenotypic resistance to tetracyclines and macrolides encoded by tet(M) and erm(B) respectively, was prevalent among Enterococcus spp. regardless of source. For E. faecium from cattle, resistance to β-lactams and quinolones was observed among 3% and 8% of isolates respectively, compared to 76% and 70% of human clinical isolates. Clinical vancomycin-resistant E. faecium exhibited high rates of multi-drug resistance, with resistance to all β-lactam, macrolides, and quinolones tested. Differences in the AMR profiles among isolates reflected antimicrobial use practices in each sector of the One-Health continuum.
Subject
- Science and technology
Keywords
- antimicrobial resistance,
- anti-infective agents,
- microbiology
Rights
Pagination
1-16
Peer review
Yes
Open access level
Gold
Identifiers
- ISSN
- 2045-2322
Article
- Journal title
- Scientific Reports
- Journal volume
- 10
- Article number
- 3937
- Accepted date
- 2020-02-13
- Submitted date
- 2019-10-19
Citation(s)
Zaheer, R., Cook, S. R., Barbieri, R., Goji, N., Cameron, A., Petkau, A., Ortega Polo, R., Tymensen, L., Stamm, C., Song, J., Hannon, S., Jones, T., Church, D., Booker, C. W., Amoako, K., Van Domselaar, G., Read, R. R., & McAllister, T. A. (2020). Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum. Scientific Reports, 10, Article 3937. https://doi.org/10.1038/s41598-020-61002-5