Extraction optimization, structural characterization, and anticoagulant activity of acidic polysaccharides from Auricularia auricula-judae
Extraction optimization, structural characterization, and anticoagulant activity of acidic polysaccharides from Auricularia auricula-judae
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Full item details
- dc.contributor.author
- Bian, Chun
- Wang, Zhenyu
- Shi, John
- dc.date.accepted
- 2020-02-04
- dc.date.accessioned
- 2025-01-14T21:32:30Z
- dc.date.available
- 2025-01-14T21:32:30Z
- dc.date.issued
- 2020-02-06
- dc.date.submitted
- 2020-01-12
- dc.description.abstract - en
- To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM). The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W). Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP). aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1. Additionally, according to the HPLC analysis, the aAAP-1 was mainly composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 80.63:9.88:2.25:1:31.13. Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.
- dc.identifier.citation
- Bian, C., Wang, Z., & Shi, J. (2020). Extraction Optimization, structural characterization, and anticoagulant activity of acidic polysaccharides from Auricularia auricula-judae. Molecules, 25(3), 710. https://doi.org/10.3390/molecules25030710
- dc.identifier.doi
- https://doi.org/10.3390/molecules25030710
- dc.identifier.issn
- 1420-3049
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3323
- dc.language.iso
- en
- dc.publisher - en
- MDPI AG
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Functional foods
- Fungi
- Science and technology
- Cardiovascular diseases
- dc.subject - fr
- Aliment fonctionnel
- Champignon
- Sciences et technologie
- Maladie cardiovasculaire
- dc.subject.en - en
- Functional foods
- Fungi
- Science and technology
- Cardiovascular diseases
- dc.subject.fr - fr
- Aliment fonctionnel
- Champignon
- Sciences et technologie
- Maladie cardiovasculaire
- dc.title - en
- Extraction optimization, structural characterization, and anticoagulant activity of acidic polysaccharides from Auricularia auricula-judae
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 710
- local.article.journalissue
- 3
- local.article.journaltitle - en
- Molecules
- local.article.journalvolume
- 25
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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