Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
- DOI
- Language of the publication
- English
- Date
- 2020-02-28
- Type
- Article
- Author(s)
- Hiebert, Colin W.
- Moscou, Matthew J.
- Hewitt, Tim
- Steuernagel, Burkhard
- Hernández-Pinzón, Inma
- Green, Phon
- Pujol, Vincent
- Zhang, Peng
- Rouse, Matthew N.
- Jin, Yue
- McIntosh, Robert A.
- Upadhyaya, Narayana
- Zhang, Jianping
- Bhavani, Sridhar
- Vrána, Jan
- Karafiátová, Miroslava
- Huang, Li
- Fetch, Tom
- Doležel, Jaroslav
- Wulff, Brande B.H.
- Lagudah, Evans
- Spielmeyer, Wolfgang
- Publisher
- Springer Nature
Alternative title
Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
Abstract
Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
Subject
- Agriculture
Keywords
- plant genetics,
- plant immunity
Peer review
Yes
Open access level
Gold
Identifiers
- ISSN
- 2041-1723
Article
- Journal title
- Nature Communications
- Journal volume
- 11
- Journal issue
- 1
Citation(s)
Hiebert, C. W., Moscou, M. J., Hewitt, T., Steuernagel, B., Hernández-Pinzón, I., Green, P., Pujol, V., Zhang, P., Rouse, M. N., Jin, Y., McIntosh, R. A., Upadhyaya, N., Zhang, J., Bhavani, S., Vrána, J., Karafiátová, M., Huang, L., Fetch, T., Doležel, J., … Spielmeyer, W. (2020). Stem rust resistance in wheat is suppressed by a subunit of the Mediator Complex. Nature Communications, 11(1). https://doi.org/10.1038/s41467-020-14937-2