Clubroot resistance gene Rcr6 in Brassica nigra resides in a genomic region homologous to chromosome A08 in B. rapa
- DOI
- Language of the publication
- English
- Date
- 2019-05-29
- Type
- Article
- Author(s)
- Chang, Adrian
- Lamara, Mebarek
- Wei, Yangdou
- Hu, Hao
- Parkin, Isobel A.P.
- Gossen, Bruce D.
- Peng, Gary
- Yu, Fengqun
- Publisher
- Springer Nature
Alternative title
Clubroot resistance gene Rcr6 in Brassica nigra resides in a genomic region homologous to chromosome A08 in B. rapa
Abstract
Clubroot, caused by Plasmodiophora brassicae Woronin, is a very important disease of Brassica species. Management of clubroot relies heavily on genetic resistance. In a cross of Brassica nigra lines PI 219576 (highly resistant, R) × CR2748 (highly susceptible, S) to clubroot, all F1 plants were resistant to clubroot. There was a 1:1 ratio of R:S in the BC1 and 3R:1S in the F2, which indicated that a single dominant gene controlled clubroot resistance in PI 219576. This gene was designated Rcr6. Mapping of Rcr6 was performed using genome sequencing information from A-genome of B. rapa and B-genome of B. nigra though bulked segregant RNA sequencing (BSR-Seq) and further mapping with Kompetitive Allele Specific PCR (KASP) analysis.
Plain language summary
N/A
Subject
- Agriculture
Keywords
- clubroot,
- bulk segregant RNA sequencing
Peer review
Yes
Open access level
Gold
Identifiers
- ISSN
- 1471-2229
Article
- Journal title
- BMC Plant Biology
- Journal volume
- 19
- Journal issue
- 1
Citation(s)
Chang, A., Lamara, M., Wei, Y., Hu, H., Parkin, I. A., Gossen, B. D., Peng, G., & Yu, F. (2019). Clubroot resistance gene RCR6 in brassica nigra resides in a genomic region homologous to chromosome a08 in B. Rapa. BMC Plant Biology, 19(1). https://doi.org/10.1186/s12870-019-1844-5