A simple and low-cost technique to initiate single-spore cultures of arbuscular mycorrhizal fungi using a superabsorbent polymer

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creativework.keywords - en

arbuscular mycorrhizal fungi

polymer

vesicular-arbuscular mycorrhizas

creativework.keywords - fr

endomycorhizes à vésicules et arbuscules

champignons mycorhiziens arbusculaires

polymère

dc.contributor.author

Paré, Louis

Banchini, Claudia

Hamel, Chantal

Bernier, Louis

Stefani, Franck

dc.date.accepted

2022-09-28

dc.date.accessioned

2024-10-10T20:02:01Z

dc.date.available

2024-10-10T20:02:01Z

dc.date.issued

2022-10-18

dc.date.submitted

2022-06-22

dc.description.abstract - en

Most species of arbuscular mycorrhizal fungi (AMF) are propagated with a host plant in a pot culture. However, the soil matrix makes it difficult to monitor the establishment and development of the symbiosis. In vitro culturing using Ri T-DNA transformed roots provides a clear medium and a sterile environment which offsets the constraints of the soil matrix. Nevertheless, the sterile conditions and the Ri T-DNA transformed roots provide very different growing conditions compared to a pot culture. Transparent soil based on superabsorbent polymer (SAP) has the potential of combining the advantages of current in vivo and in vitro culture methods without the constraints associated with either technique (opacity and sterility). Here we describe a SAP-based autotrophic culture as an alternative to current in vivo and in vitro culture methods. This system using two-compartment Petri dishes makes it easy to initiate single-spore cultures and to monitor fungal propagation. The SAP-based autotrophic system allowed the establishment of single-spore cultures of seven species (Diversispora varaderana, Funneliformis geosporus, Gigaspora rosea, Racocetra fulgida, Rhizophagus irregularis, R. intraradices and Sclerocystis sp.) from six genera and three families. Cultures were maintained over several months under non-sterile conditions. The Petri dishes avoid the problem of cross contamination and they can be stacked for space optimization. The grains of SAP colonized with new spores were used as inoculum to initiate new cultures in the SAP-based system. The SAP-based autotrophic culture method is a low-cost and low-tech approach, which makes the study of AMF much more accessible.

dc.identifier.citation

Paré, L., Banchini, C., Hamel, C., Bernier, L. & Stefani, F. (2022). A simple and low-cost technique to initiate single-spore cultures of arbuscular mycorrhizal fungi using a superabsorbent polymer. Symbiosis 88(1-3), 61–73. https://doi.org/10.1007/s13199-022-00878-5

dc.identifier.doi

https://doi.org/10.1007/s13199-022-00878-5

dc.identifier.issn

1878-7665

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/3045

dc.language.iso

en

dc.publisher

Springer Nature

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Nature and environment

Science and technology

dc.subject - fr

Nature et environnement

Sciences et technologie

dc.subject.en - en

Nature and environment

Science and technology

dc.subject.fr - fr

Nature et environnement

Sciences et technologie

dc.title - en

A simple and low-cost technique to initiate single-spore cultures of arbuscular mycorrhizal fungi using a superabsorbent polymer

dc.type - en

Article

dc.type - fr

Article

local.article.journalissue

1-3

local.article.journaltitle

Symbiosis

local.article.journalvolume

88

local.pagination

61-73

local.peerreview - en

Yes

local.peerreview - fr

Oui

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