Genetic variation and structural diversity in major seed proteins among and within Camelina species

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creativework.keywords - en
false flax
gene expression
protein functionality
protein modelling
creativework.keywords - fr
faux lin
expression génique
fonctionnalité des proteïnes
modélisation des proteïnes
dc.contributor.author
Hegedus, Dwayne
Coutu, Cathy
Gjetvaj, Branimir
Hannoufa, Abdelali
Harrington, Myrtle
Martin, Sara
Parkin, Isobel A. P.
Perera, Suneru
Wanasundara, Janitha
dc.date.accepted
2022-09-12
dc.date.accessioned
2024-10-10T20:21:10Z
dc.date.available
2024-10-10T20:21:10Z
dc.date.issued
2022-10-06
dc.date.submitted
2022-04-08
dc.description.abstract - en
Seed protein diversity was examined in six Camelina species (C. hispida, C. laxa, C. microcarpa, C. neglecta, C. rumelica and C. sativa). Differences were observed in seed protein electrophoretic profiles, total seed protein content and amino acid composition between the species. Genes encoding major seed proteins (cruciferins, napins, oleosins and vicilins) were catalogued for C. sativa and RNA-Seq analysis established the expression patterns of these and other genes in developing seed from anthesis through to maturation. Examination of 187 C. sativa accessions revealed limited variation in seed protein electrophoretic profiles, though sufficient to group the majority into classes based on high MW protein profiles corresponding to the cruciferin region. C. sativa possessed four distinct types of cruciferins, named CsCRA, CsCRB, CsCRC and CsCRD, which corresponded to orthologues in Arabidopsis thaliana with members of each type encoded by homeologous genes on the three C. sativa sub-genomes. Total protein content and amino acid composition varied only slightly; however, RNA-Seq analysis revealed that CsCRA and CsCRB genes contributed > 95% of the cruciferin transcripts in most lines, whereas CsCRC genes were the most highly expressed cruciferin genes in others, including the type cultivar DH55. This was confirmed by proteomics analyses. Cruciferin is the most abundant seed protein and contributes the most to functionality. Modelling of the C. sativa cruciferins indicated that each type possesses different physiochemical attributes that were predicted to impart unique functional properties. As such, opportunities exist to create C. sativa cultivars with seed protein profiles tailored to specific technical applications.
dc.identifier.citation
Hegedus, D., Coutu, C., Gjetvaj, B., Hannoufa, A., Harrington, M., Martin, S., Parkin, I. A. P., Perera, S., & Wanasundara, J. (2022). Genetic variation and structural diversity in major seed proteins among and within Camelina species. Planta 256, 93. https://doi.org/10.1007/s00425-022-03998-w
dc.identifier.doi
https://doi.org/10.1007/s00425-022-03998-w
dc.identifier.issn
1432-2048
dc.identifier.uri
https://open-science.canada.ca/handle/123456789/3046
dc.language.iso
en
dc.publisher
Springer Nature
dc.rights - en
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.uri - en
https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr
https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en
Nature and environment
Science and technology
dc.subject - fr
Nature et environnement
Sciences et technologie
dc.subject.en - en
Nature and environment
Science and technology
dc.subject.fr - fr
Nature et environnement
Sciences et technologie
dc.title - en
Genetic variation and structural diversity in major seed proteins among and within Camelina species
dc.type - en
Article
dc.type - fr
Article
local.acceptedmanuscript.articlenum
93
local.article.journaltitle
Planta
local.article.journalvolume
256
local.pagination
23 pages
local.peerreview - en
Yes
local.peerreview - fr
Oui
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