Structural properties of cruciferin and napin of Brassica napus (canola) show distinct responses to changes in pH and temperature

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creativework.keywords - en

Canola--Effect of temperature on

Plant proteins

Plants--Effect of hydrogen-ion concentration on

creativework.keywords - fr

Colza canola--Effets de la température sur

Protéines végétales

Plantes--Effets du pH sur

dc.contributor.author

Perera, Suneru P.

McIntosh, Tara C.

Wanasundara, Janitha P. D.

dc.date.accepted

2016-08-25

dc.date.accessioned

2025-10-20T13:36:52Z

dc.date.available

2025-10-20T13:36:52Z

dc.date.issued

2016-09-07

dc.date.submitted

2016-03-15

dc.description.abstract - en

The two major storage proteins identified in Brassica napus (canola) were isolated and studied for their molecular composition, structural characteristics and the responses of structural features to the changes in pH and temperature. Cruciferin, a complex of six monomers, has a predominantly β-sheet-containing secondary structure. This protein showed low pH unstable tertiary structure, and distinctly different solubility behaviour with pH when intact in the seed cellular matrix. Cruciferin structure unfolds at pH 3 even at ambient temperature. Temperature-induced structure unfolding was observed above the maximum denaturation temperature of cruciferin. Napin was soluble in a wider pH range than cruciferin and has α-helices dominating secondary structure. Structural features of napin showed less sensitivity to the changes in medium pH and temperature. The surface hydrophobicity (S0) and intrinsic fluorescence of tryptophan residue appear to be good indicators of cruciferin unfolding, however they were not the best to demonstrate structural changes of napin. These two storage proteins of B. napus have distinct molecular characteristics, therefore properties and functionalities they provide are contrasting rather than complementary.

dc.description.fosrcfull - en

This article belongs to the Special Issue: Selected/Extended Full Papers of 14th International Rapeseed Congress

dc.description.fosrcfull-fosrctranslation - fr

Cet article fait partie du numéro spécial: Selected/Extended Full Papers of 14th International Rapeseed Congress.

dc.identifier.citation

Perera, S. P., McIntosh, T. C., & Wanasundara, J. P. D. (2016). Structural properties of cruciferin and napin of Brassica napus (canola) show distinct responses to changes in pH and temperature. Plants, 5(3), Article 36. https://doi.org/10.3390/plants5030036

dc.identifier.doi

https://doi.org/10.3390/plants5030036

dc.identifier.issn

2223-7747

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/3970

dc.language.iso

en

dc.publisher - en

MDPI

dc.publisher - fr

MDPI

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.openaccesslevel - en

Gold

dc.rights.openaccesslevel - fr

Or

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Crops

dc.subject - fr

Cultures

dc.subject.en - en

Crops

dc.subject.fr - fr

Cultures

dc.title - en

Structural properties of cruciferin and napin of Brassica napus (canola) show distinct responses to changes in pH and temperature

dc.type - en

Article

dc.type - fr

Article

local.acceptedmanuscript.articlenum

36

local.article.journalissue

3

local.article.journaltitle - en

Plants

local.article.journalvolume

5

local.pagination

1-24

local.peerreview - en

Yes

local.peerreview - fr

Oui

local.requestdoi - en

No

local.requestdoi - fr

No

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