A plant RNA virus activates selective autophagy in a UPR-dependent manner to promote virus infection
A plant RNA virus activates selective autophagy in a UPR-dependent manner to promote virus infection
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- creativework.keywords - en
- autophagy
- potyvirus
- RNA-dependent RNA polymerase
- tonoplast
- turnip mosaic virus
- unfolded protein response
- plant vacuoles
- virus replication complex
- creativework.keywords - fr
- autophagie
- potyvirus
- ARN polymérase ARN dépendante
- tonoplaste
- virus de la mosaïque du navet
- réponse de la protéine dépliée
- plantes--vacuoles
- complexe de réplication virale
- dc.contributor.author
- Fangfang, Li
- Zhang, Changwei
- Tang, Ziwei
- Zhang, Lingrui
- Dai, Zhaoji
- Lyu, Shanwu
- Li, Yinzi
- Hou, Xilin
- Bernards, Mark
- Wang, Aiming
- dc.date.accessioned
- 2023-04-12T17:36:50Z
- dc.date.available
- 2023-04-12T17:36:50Z
- dc.date.issued
- 2020-09-23
- dc.description.abstract - en
- Autophagy is an evolutionarily conserved pathway in eukaryotes that delivers unwanted cytoplasmic materials to the lysosome/vacuole for degradation/recycling. Stimulated autophagy emerges as an integral part of plant immunity against intracellular pathogens. In this study, we used turnip mosaic virus (TuMV) as a model to investigate the involvement of autophagy in plant RNA virus infection. The small integral membrane protein 6K2 of TuMV, known as a marker of the virus replication site and an elicitor of the unfolded protein response (UPR), upregulates the selective autophagy receptor gene NBR1 in a UPR-dependent manner. NBR1 interacts with TuMV NIb, the RNA-dependent RNA polymerase of the virus replication complex (VRC), and the autophagy cargo receptor/adaptor protein ATG8f. The NIb/NBR1/ATG8f interaction complexes colocalise with the 6K2-stained VRC. Overexpression of NBR1 or ATG8f enhances TuMV replication, and deficiency of NBR1 or ATG8f inhibits virus infection. In addition, ATG8f interacts with the tonoplast-specific protein TIP1 and the NBR1/ATG8f-containing VRC is enclosed by the TIP1-labelled tonoplast. In TuMV-infected cells, numerous membrane-bound viral particles are evident in the vacuole. Altogether these results suggest that TuMV activates and manipulates UPR-dependent NBR1-ATG8f autophagy to target the VRC to the tonoplast to promote viral replication and virion accumulation.
- dc.identifier.citation
- Li, F., Zhang, C., Tang, Z., Zhang, L., Dai, Z., Lyu, S., Li, Y., Hou, X., Bernards, M., & Wang, A. (2020). A plant RNA virus activates selective autophagy in a upr‐dependent manner to promote virus infection. New Phytologist, 228(2), 622–639. https://doi.org/10.1111/nph.16716
- dc.identifier.doi
- https://doi.org/10.1111/nph.16716
- dc.identifier.issn
- 1469-8137
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/134
- dc.language.iso
- en
- dc.publisher
- Wiley
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.subject - en
- Agriculture
- dc.subject - fr
- Agriculture
- dc.subject.en - en
- Agriculture
- dc.subject.fr - fr
- Agriculture
- dc.title - en
- A plant RNA virus activates selective autophagy in a UPR-dependent manner to promote virus infection
- dc.title.fosrctranslation - en
- A plant RNA virus activates selective autophagy in a UPR-dependent manner to promote virus infection
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journalissue
- 2
- local.article.journaltitle
- New Phytologist
- local.article.journalvolume
- 228
- local.article.pagination
- 622-639
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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