Effect of probiotic bacteria on porcine rotavirus OSU infection of porcine intestinal epithelial IPEC-J2 cells
Effect of probiotic bacteria on porcine rotavirus OSU infection of porcine intestinal epithelial IPEC-J2 cells
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Full item details
- creativework.keywords - en
- pigs
- probiotic bacteria
- retrovirus infections
- creativework.keywords - fr
- porcs
- bactéries probiotiques
- infections à retrovirus
- dc.contributor.author
- Leblanc, Danielle
- Raymond, Yves
- Lemay, Marie-Josée
- Champagne, Claude P.
- Brassard, Julie
- dc.date.accepted
- 2022-05-10
- dc.date.accessioned
- 2024-10-28T21:42:08Z
- dc.date.available
- 2024-10-28T21:42:08Z
- dc.date.issued
- 2022-07-06
- dc.date.submitted
- 2022-01-25
- dc.description.abstract - en
- Rotavirus infections in nursing or post-weaning piglets are known to cause diarrhea, which can lead to commercial losses. Probiotic supplementation is used as a prophylactic or therapeutic approach to dealing with microbial infections in humans and animals. To evaluate the effect of probiotic bacteria on porcine rotavirus infections, non-transformed porcine intestinal epithelial IPEC-J2 cells were used as an in vitro model, and three different procedures were tested. When cells were exposed to seven probiotics at concentrations of 105, 106, or 107 CFU/mL for 16 h and removed before rotavirus challenge, infection reduction rates determined by flow cytometry were as follows: 15% (106) and 18% (105) for Bifidobacterium longum R0175, 15% (107) and 16% (106) for B. animalis lactis A026, and 15% (105) for Lactobacillus plantarum 299V. When cells were exposed to three selected probiotic strains for 1 h at higher concentrations, that is, 108 and 5 × 108 CFU/mL, before infection with rotavirus, no significant reduction was observed. When the probiotic bacteria were incubated with the virus before cell infection, a significant 14% decrease in the infection rate was observed for B. longum R0175. The results obtained using a cell-probiotics-virus platform combined with flow cytometry analysis suggest that probiotic bacteria can have a protective effect on IPEC-J2 cells before infection and can also prevent rotavirus infection of the cells.
- dc.identifier.citation
- Leblanc, D., Raymond, Y., Lemay, M-J., Champagen, C. P., & Brassard, J. (2022). Effect of probiotic bacteria on porcine rotavirus OSU infection of porcine intestinal epithelial IPEC-J2 cells. Archives of Virology 167, 1999–2010. https://doi.org/10.1007/s00705-022-05510-x
- dc.identifier.doi
- https://doi.org/10.1007/s00705-022-05510-x
- dc.identifier.issn
- 1432-8798
- 0304-8608
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3088
- dc.language.iso
- en
- dc.publisher
- Springer Nature
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Agriculture
- dc.subject - fr
- Agriculture
- dc.subject.en - en
- Agriculture
- dc.subject.fr - fr
- Agriculture
- dc.title - en
- Effect of probiotic bacteria on porcine rotavirus OSU infection of porcine intestinal epithelial IPEC-J2 cells
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journaltitle
- Archives of Virology
- local.article.journalvolume
- 167
- local.pagination
- 1999-2010
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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