Morphological and molecular characterization of Macrophomina phaseolina isolated from three legume crops and evaluation of mungbean genotypes for resistance to dry root rot

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dc.contributor.author

Pandey, Abhay K.

Burlakoti, Rishi R.

Rathore, Abhishek

Nair, Ramakrishnan M.

dc.date.accepted

2019-09-16

dc.date.accessioned

2024-06-24T17:52:22Z

dc.date.available

2024-06-24T17:52:22Z

dc.date.issued

2019-09-18

dc.date.submitted

2018-12-24

dc.description.abstract - en

Dry root rot (DRR) is an important emerging disease of mungbean caused by fungus Macrophomina phaseolina. The disease is seed and soil-borne and hence management is difficult. Exploitation of host resistance could be a good option to manage the disease. The objectives of this study were to characterize the isolates of M. phaseolina from three legume crops and to identify resistant sources against DRR of mungbean. Isolates of M. phaseolina from mungbean, urdbean, and vegetable soybean were identified using morphological characteristics and sequencing internal transcribed sequence (ITS) region of 18S rRNA. Isolates of M. phaseolina from urdbean and vegetable soybean were inoculated on susceptible check genotype of mungbean to assess their pathogenicity. Forty three mungbean genotypes were screened against M. phaseolina isolate of mungbean using the paper towel method to identify sources of resistance. Among these genotypes, 9 were resistant in repeated experiment, with disease score ranging from 1.9 to 3.0. Resistant genotypes showing lower disease score, namely IPM99-125, EC693368, and EC693369, were further screened using the sick pot method to confirm their resistance. Among these three genotypes, IPM99-125 showed consistently higher plant survival rate followed by EC693368 and EC693369 as compared to susceptible checks (VC3960-88, KPS1). In addition, these three genotypes were resistant when screened with two strains of M. phaseolina isolated from urdbean and vegetable soybean, with IPM99-125 having lower disease score. The mungbean genotype IPM99-125 could be useful in mungbean breeding programs to develop root rot-resistant varieties.

dc.description.fosrcfull - en

Published first online in 2019, and then published in print in 2020.

dc.identifier.citation

Pandey, A. K., Burlakoti, R. R., Rathore, A., & Nair, R. M. (2020). Morphological and molecular characterization of macrophomina phaseolina isolated from three legume crops and evaluation of mungbean genotypes for resistance to dry root rot. Crop Protection, 127, Article 104962. https://doi.org/10.1016/j.cropro.2019.104962

dc.identifier.doi

https://doi.org/10.1016/j.cropro.2019.104962

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/2633

dc.language.iso

en

dc.publisher

Elsevier Ltd

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.openaccesslevel - en

Gold

dc.rights.openaccesslevel - fr

Or

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Agriculture

dc.subject - fr

Agriculture

dc.subject.en - en

Agriculture

dc.subject.fr - fr

Agriculture

dc.title - en

Morphological and molecular characterization of Macrophomina phaseolina isolated from three legume crops and evaluation of mungbean genotypes for resistance to dry root rot

dc.type - en

Article

dc.type - fr

Article

local.acceptedmanuscript.articlenum

104962

local.article.journaltitle

Crop Protection

local.article.journalvolume

127

local.peerreview - en

Yes

local.peerreview - fr

Oui

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