Whole genome sequencing and phylogenomic analysis show support for the splitting of genus Pythium

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DOI

https://doi.org/10.1080/00275514.2022.2045116

Language of the publication
English
Date
2022-05-06
Type
Article
Author(s)
  • Nguyen, Hai D. T.
  • Dodge, Annette
  • Dadej, Kasia
  • Rintoul, Tara L.
  • Ponomareva, Ekaterina
  • Martin, Frank N.
  • de Cock, Arthur W. A. M.
  • Lévesque, C. André
  • Redhead, Scott A.
  • Spies, Christoffel F. J.
Publisher
Taylor and Francis

Abstract

The genus Pythium (nom. cons.) sensu lato (s.l.) is composed of many important species of plant pathogens. Early molecular phylogenetic studies suggested paraphyly of Pythium, which led to a formal proposal by Uzuhashi and colleagues in 2010 to split the genus into Pythium sensu stricto (s.s.), Elongisporangium, Globisporangium, Ovatisporangium (= Phytopythium), and Pilasporangium using morphological characters and phylogenies of the mt cytochrome c oxidase subunit 2 (cox2) and D1–D2 domains of nuc 28S rDNA. Although the split was fairly justified by the delineating morphological characters, there were weaknesses in the molecular analyses, which created reluctance in the scientific community to adopt these new genera for the description of new species. In this study, this issue was addressed using phylogenomics. Whole genomes of 109 strains of Pythium and close relatives were sequenced, assembled, and annotated. These data were combined with 10 genomes sequenced in previous studies. Phylogenomic analyses were performed with 148 single-copy genes represented in at least 90% of the taxa in the data set. The results showed support for the division of Pythium s.l. The status of alternative generic names that have been used for species of Pythium in the past (e.g., Artotrogus, Cystosiphon, Eupythium, Nematosporangium, Rheosporangium, Sphaerosporangium) was investigated. Based on our molecular analyses and review of the Pythium generic concepts, we urge the scientific community to adopt the generic names Pythium, Elongisporangium, Globisporangium, and their concepts as proposed by Uzuhashi and colleagues in 2010 in their work going forward. In order to consolidate the taxonomy of these genera, some of the recently described Pythium spp. are transferred to Elongisporangium and Globisporangium.

Subject

  • Nature and environment

Keywords

  • Biology--Classification,
  • Biologie--Classification,
  • Oomycètes,
  • Oomycetes,
  • Séquençage du génome entier

Rights

Pagination

501-515

Peer review

Yes

Open access level

Gold

Identifiers

ISSN
0027-5514
1557-2536

Article

Journal title
Mycologia
Journal volume
114
Journal issue
3
Accepted date
2022-02-18
Submitted date
2021-09-13

Citation(s)

Nguyen, H. D. T., Dodge, A., Dadej, K., Rintoul, T. L., Ponomareva, E., Martin, F. N., de Cock, A. W. A. M., Lévesque, C. A., Redhead, S. A., & Spies, C. F. J. (2022). Whole genome sequencing and phylogenomic analysis show support for the splitting of genus Pythium. Mycologia, 114(3), 501-515. https://doi.org/10.1080/00275514.2022.2045116

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Collection(s)

Biology

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