Evaluation of a lateral flow assay for rapid detection of African Swine Fever virus in multiple sample types

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creativework.keywords - en
African swine fever
African and classical swine fever
ASF
CSF
creativework.keywords - fr
Peste porcine africaine
Peste porcine africaine et classique
PPA
PPC
dc.contributor.author
Onyilagha, Chukwunonso
Nguyen, Kevin
Luka, Pam D.
Hussaini, Ularamu
Adedeji, Adeyinka
Odoom, Theophilus
Ambagal, Aruna
dc.date.accepted
2022-01-19
dc.date.accessioned
2025-03-14T17:15:19Z
dc.date.available
2025-03-14T17:15:19Z
dc.date.issued
2022-01-24
dc.date.submitted
2021-12-21
dc.description.abstract - en
Antibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect pathogens in field samples, especially in hard-to-reach remote areas that may have limited access to central laboratories during an outbreak or surveillance. In this study, we investigated the ability of a commercially available LFA, PenCheck®, to detect African swine fever virus (ASFV) in clinical samples derived from pigs infected with highly virulent ASFV strains. The assay was specific and positively identified the majority of pigs showing high fever during the early stages (between 3 and 5 days) of infection. PenCheck® LFA also detected ASFV in serum and tissue samples collected from pigs that succumbed to experimental ASFV infection and whole blood, plasma, and tissue samples from the field. The limit of detection of the assay was ASFV titer 107.80 TCID50/mL, corresponding to ASFV real-time PCR values below 23 Ct. Although the sensitivity of the assay is less than that of the laboratory-based real-time PCR assays, the results obtained with the PenCheck® LFA in this study suggest that it can be used as a herd-level, field-deployable, and easy-to-use diagnostic tool to identify ASF-affected farms when access to portable molecular assays or central laboratories is not possible.
dc.identifier.citation
Onyilagha, C., Nguyen, K., Luka, P. D., Hussaini, U., Adedeji, A., Odoom, T., & Ambagala, A. (2022, January 24). Evaluation of a lateral flow assay for rapid detection of African swine fever virus in multiple sample types. MDPI. https://doi.org/10.3390/pathogens11020138
dc.identifier.doi
https://doi.org/10.3390/pathogens11020138
dc.identifier.uri
https://open-science.canada.ca/handle/123456789/3518
dc.language.iso
en
dc.publisher - en
MDPI
dc.rights - en
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.uri - en
https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr
https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en
Animal diseases
dc.subject - fr
Maladie animale
dc.subject.en - en
Animal diseases
dc.subject.fr - fr
Maladie animale
dc.title - en
Evaluation of a lateral flow assay for rapid detection of African Swine Fever virus in multiple sample types
dc.type - en
Article
dc.type - fr
Article
local.acceptedmanuscript.articlenum
138
local.article.journalissue
2
local.article.journaltitle - en
Pathogens
local.article.journalvolume
11
local.peerreview - en
Yes
local.peerreview - fr
Oui
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