Verification and use of the US-FDA BAM 19b method for detection of Cyclospora cayetanensis in a survey of fresh produce by CFIA laboratory
Verification and use of the US-FDA BAM 19b method for detection of Cyclospora cayetanensis in a survey of fresh produce by CFIA laboratory
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- creativework.keywords - en
- Food safety
- creativework.keywords - fr
- Sécurité alimentaire
- dc.contributor.author
- Lalonde, Laura
- Oakley, Jenna
- Fries, Patrick
- dc.date.accessioned
- 2023-05-19T13:37:15Z
- dc.date.available
- 2023-05-19T13:37:15Z
- dc.date.issued
- 2022-03-04
- dc.description.abstract - en
- To facilitate the harmonized surveillance and investigation of cyclosporiasis outbreaks in the US and Canada, we adapted and verified the US-FDA’s BAM 19b method and employed it in a national produce survey. Performance was verified by spiking 200, 10, 5 or 0 C. cayetanensis oocysts onto berries (50 ± 5 g, n = 85) and 200, 10 or 0 oocysts onto green onions (25 ± 3 g, n = 24) and leafy greens (25 ± 1 g, n = 120) and testing these samples by the BAM method on Bio-Rad CFX96. Method robustness was assessed by aging (0 or 7 days) and freezing the produce and washes prior to testing, then implementing the method for the surveillance testing of 1759 imported leafy green, herb and berry samples. Diagnostic sensitivity was 100/44% and 93/30% for berries and leafy greens spiked with 200/10 oocysts, respectively. The diagnostic and analytical specificity were 100% for all matrices and related parasites tested. The proportion positive was unaffected (p = 0.22) by age or condition of produce (7d, fresh, frozen) or wash concentrate (3d, fresh, frozen); however, the Cq values were higher (p = 0.009) for raspberries aged 7d (37.46 ± 0.29) compared to fresh (35.36 ± 0.29). C. cayetanensis was detected in berries (two), herbs (two) and leafy greens (one), representing 0.28% of the tested survey samples. These results independently verified the reported performance characteristics and robustness of the BAM method for the detection of C. cayetanensis in a variety of matrices, including under adverse sample conditions, using a unique detection platform and demonstrating its routine diagnostic use in our Canadian Food Inspection Agency (CFIA) laboratory.
- dc.identifier.citation
- Lalonde, L., Oakley, J., & Fries, P. (2022). Verification and use of the US-FDA BAM 19b method for detection of Cyclospora cayetanensis in a survey of fresh produce by CFIA laboratory. Microorganisms, 10(3), Article 559. https://doi.org/10.3390/microorganisms10030559
- dc.identifier.doi
- https://doi.org/10.3390/microorganisms10030559
- dc.identifier.issn
- 2076-2607
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/289
- dc.language.iso
- en
- dc.publisher
- MDPI
- dc.rights - en
- Creative Commons Public Domain Dedication (CC0 1.0 Universal)
- dc.rights - fr
- Creative Commons Transfert dans le Domaine Public (CC0 1.0 universel)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/publicdomain/zero/1.0/
- dc.rights.uri - fr
- https://creativecommons.org/publicdomain/zero/1.0/deed.fr
- dc.subject - en
- Agriculture
- dc.subject - fr
- Agriculture
- dc.subject.en - en
- Agriculture
- dc.subject.fr - fr
- Agriculture
- dc.title - en
- Verification and use of the US-FDA BAM 19b method for detection of Cyclospora cayetanensis in a survey of fresh produce by CFIA laboratory
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journalissue
- 3
- local.article.journaltitle
- Microorganisms
- local.article.journalvolume
- 10
- local.pagination
- 13 pages
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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