A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (Glycine max)
A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (Glycine max)
Simple item page
Full item details
- dc.contributor.author
- Anguraj Vadivel, Arun Kumaran
- McDowell, Tim
- Renaud, Justin B.
- Dhaubhadel, Sangeeta
- dc.date.accepted
- 2021-02-19
- dc.date.accessioned
- 2024-08-21T13:50:28Z
- dc.date.available
- 2024-08-21T13:50:28Z
- dc.date.issued
- 2021-03-19
- dc.date.submitted
- 2020-09-03
- dc.description.abstract - en
- GmMYB176 is an R1 MYB transcription factor that regulates multiple genes in the isoflavonoid biosynthetic pathway, thereby affecting their levels in soybean roots. While GmMYB176 is important for isoflavonoid synthesis, it is not sufficient for the function and requires additional cofactor(s). The aim of this study was to identify the GmMYB176 interactome for the regulation of isoflavonoid biosynthesis in soybean. Here, we demonstrate that a bZIP transcription factor GmbZIP5 co-immunoprecipitates with GmMYB176 and shows protein–protein interaction in planta. RNAi silencing of GmbZIP5 reduced the isoflavonoid level in soybean hairy roots. Furthermore, co-overexpression of GmMYB176 and GmbZIP5 enhanced the level of multiple isoflavonoid phytoallexins including glyceollin, isowighteone and a unique O-methylhydroxy isoflavone in soybean hairy roots. These findings could be utilized to develop biotechnological strategies to manipulate the metabolite levels either to enhance plant defense mechanisms or for human health benefits in soybean or other economically important crops.
- dc.identifier.citation
- Anguraj Vadivel, A. K., McDowell, T., Renaud, J. B., & Dhaubhadel, S. (2021). A combinatorial action of GMMYB176 and GMBZIP5 controls isoflavonoid biosynthesis in soybean (glycine max). Communications Biology, 4, Article 356. https://doi.org/10.1038/s42003-021-01889-6
- dc.identifier.doi
- https://doi.org/10.1038/s42003-021-01889-6
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/2867
- dc.language.iso
- en
- dc.publisher
- Nature Research
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Agriculture
- dc.subject - fr
- Agriculture
- dc.subject.en - en
- Agriculture
- dc.subject.fr - fr
- Agriculture
- dc.title - en
- A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (Glycine max)
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 356
- local.article.journaltitle
- Communications Biology
- local.article.journalvolume
- 4
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
Download(s)
Original bundle
1 - 1 of 1
Name: AcombinatorialactionofGmMYB176andGmbZIP5_2021.pdf
Size: 2.55 MB
Format: PDF
Collection(s)