RNA decay is an antiviral defense in plants that is counteracted by viral RNA silencing suppressors
RNA decay is an antiviral defense in plants that is counteracted by viral RNA silencing suppressors
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- creativework.keywords - en
- Antiviral agents
- Antiviraux
- Arabidopsis
- Arabidopsis
- Plants--Disease and pest resistance
- Plantes--Résistance aux maladies et fléaux
- Gene regulation
- Régulation génétique
- creativework.keywords - fr
- RNA interference
- Interférence ARN
- Petits ARN interférents
- Small interfering RNA
- Plant diseases
- Plantes--Maladies et fléaux
- Potyviruses
- Potyvirus
- dc.contributor.author
- Li, Fangfang
- Wang, Aiming
- dc.date.accepted
- 2018-07-17
- dc.date.accessioned
- 2024-07-12T14:42:32Z
- dc.date.available
- 2024-07-12T14:42:32Z
- dc.date.issued
- 2018-08-03
- dc.date.submitted
- 2018-04-17
- dc.description.abstract - en
- Exonuclease-mediated RNA decay in plants is known to be involved primarily in endogenous RNA degradation, and several RNA decay components have been suggested to attenuate RNA silencing possibly through competing for RNA substrates. In this paper, we report that overexpression of key cytoplasmic 5’–3’ RNA decay pathway gene-encoded proteins (5’RDGs) such as decapping protein 2 (DCP2) and exoribonuclease 4 (XRN4) in Nicotiana benthamiana fails to suppress sense transgene-induced post-transcriptional gene silencing (S-PTGS). On the contrary, knock-down of these 5’RDGs attenuates S-PTGS and supresses the generation of small interfering RNAs (siRNAs). We show that 5’RDGs degrade transgene transcripts via the RNA decay pathway when the S-PTGS pathway is disabled. Thus, RNA silencing and RNA decay degrade exogenous gene transcripts in a hierarchical and coordinated manner. Moreover, we present evidence that infection by turnip mosaic virus (TuMV) activates RNA decay and 5’RDGs also negatively regulate TuMV RNA accumulation. We reveal that RNA silencing and RNA decay can mediate degradation of TuMV RNA in the same way that they target transgene transcripts. Furthermore, we demonstrate that VPg and HC-Pro, the two known viral suppressors of RNA silencing (VSRs) of potyviruses, bind to DCP2 and XRN4, respectively, and the interactions compromise their antiviral function. Taken together, our data highlight the overlapping function of the RNA silencing and RNA decay pathways in plants, as evidenced by their hierarchical and concerted actions against exogenous and viral RNA, and VSRs not only counteract RNA silencing but also subvert RNA decay to promote viral infection.
- dc.identifier.citation
- Li, F., & Wang, A. (2018). RNA decay is an antiviral defense in plants that is counteracted by viral RNA silencing suppressors. PLoS Pathogens, 14(8), Article e1007228. https://doi.org/10.1371/journal.ppat.1007228
- dc.identifier.doi
- https://doi.org/10.1371/journal.ppat.1007228
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/2683
- dc.language.iso
- en
- dc.publisher
- Public Library of Science
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Nature and environment
- Science and technology
- dc.subject - fr
- Nature et environnement
- Sciences et technologie
- dc.subject.en - en
- Nature and environment
- Science and technology
- dc.subject.fr - fr
- Nature et environnement
- Sciences et technologie
- dc.title - en
- RNA decay is an antiviral defense in plants that is counteracted by viral RNA silencing suppressors
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- e1007228
- local.article.journalissue
- 8
- local.article.journaltitle
- PLoS Pathogens
- local.article.journalvolume
- 14
- local.pagination
- 1-32
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
- local.requestdoi
- No
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