Bacterial and fungal core microbiomes associated with small grain silages during ensiling and aerobic spoilage

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dc.contributor.author

Duniere, Lysiane

Xu, Shanwei

Long, Jin

Elekwachi, Chijioke

Wang, Yuxi

Turkington, Kelly

Forster, Robert

McAllister, Tim A.

dc.date.accepted

2017-02-07

dc.date.accessioned

2024-01-08T16:36:12Z

dc.date.available

2024-01-08T16:36:12Z

dc.date.issued

2017-03-03

dc.date.submitted

2016-10-09

dc.description.abstract - en

Background Describing the microbial populations present in small grain silage and understanding their changes during ensiling is of interest for improving the nutrient value of these important forage crops. Barley, oat and triticale forages as well as an intercropped mixture of the 3 crops were harvested and ensiled in mini silos for a period of 90 days, followed by 14 days of aerobic exposure. Changes in fermentation characteristics and nutritive value were assessed in terminal silages and bacterial and fungal communities during ensiling and aerobic exposure were described using 16S and 18S rDNA sequencing, respectively. Results All small grain silages exhibited chemical traits that were associated with well ensiled forages, such as low pH value (4.09 ± 0.28) and high levels of lactic acid (59.8 ± 14.59 mg/g DM). The number of microbial core genome operational taxonomic units (OTUs) decreased with time of ensiling. Taxonomic bacterial community profiles were dominated by the Lactobacillales after fermentation, with a notable increase in Bacillales as a result of aerobic exposure. Diversity of the fungal core microbiome was shown to also be reduced during ensiling. Operational taxonomic units assigned to filamentous fungi were found in the core microbiome at ensiling and after aerobic exposure, whereas the Saccharomycetales were the dominate yeast population after 90 days of ensiling and aerobic exposure. Bacterial and fungal orders typically associated with silage spoilage were identified in the core microbiome after aerobic exposure. Conclusion Next Generation Sequencing was successfully used to describe bacterial communities and the first record of fungal communities throughout the process of ensiling and utilization. Adequately describing the microbial ecology of silages could lead to improved ensiling practices and the selection of silage inoculants that act synergistically with the natural forage microbiome.

dc.identifier.citation

Duniere, L., Xu, S., Long, J., Elekwachi, C., Wang, Y., Turkington, K., Forster, R., & McAllister, T. A. (2017). Bacterial and fungal core microbiomes associated with small grain silages during ensiling and aerobic spoilage. BMC Microbiology, 17, Article 50. https://doi.org/10.1186/s12866-017-0947-0

dc.identifier.doi

https://doi.org/10.1186/s12866-017-0947-0

dc.identifier.issn

1471-2180

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/1546

dc.language.iso

en

dc.publisher

BioMed Central Ltd.

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.openaccesslevel - en

Gold

dc.rights.openaccesslevel - fr

Or

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Agriculture

dc.subject - fr

Agriculture

dc.subject.en - en

Agriculture

dc.subject.fr - fr

Agriculture

dc.title - en

Bacterial and fungal core microbiomes associated with small grain silages during ensiling and aerobic spoilage

dc.type - en

Article

dc.type - fr

Article

local.acceptedmanuscript.articlenum

50

local.article.journaltitle

BMC Microbiology

local.article.journalvolume

17

local.pagination

1-16

local.peerreview - en

Yes

local.peerreview - fr

Oui

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