Detection of anticoagulant rodenticides by direct analysis in real time time-of-flight mass spectrometry : novel screening techniques and rapid semi-quantitative determination

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dc.contributor.author
Yu, Trista
Moorthy, Arun S.
Brunswick, Pamela
Rosary, Bianca
Cody, Robert
Gian, Marcus
Dang, Vanessa
Kwok, Honoria
Yan, Jeffrey
Shang, Dayue
dc.date.accepted
2025-10-12
dc.date.accessioned
2026-02-10T14:17:56Z
dc.date.available
2026-02-10T14:17:56Z
dc.date.issued
2025-10-23
dc.date.submitted
2025-08-29
dc.description.abstract - en
Environmental monitoring of rodenticide contamination is often required to address public concern of off-target poisoning, which threatens animal populations and disrupts related ecology. This study aimed to address the shortcomings of current analytical methods for testing water-soluble anticoagulant rodenticide by application of direct analysis in real time in tandem with time-of-flight (DART-ToF) mass spectrometry (MS), an alternative analytical technique offering rapidity, low cost, and robustness. Both positive and negative ion modes were investigated with the aim of establishing a quick screening and semi-quantitation workflow. Screening was conducted by development of a custom DART-ToF MS library with selected rodenticide spectra, and data was analyzed using the National Institute of Standards and Technology/National Institute of Justice DART-MS Data Interpretation Tool (NIST/NIJ DART-MS DIT). The developed methodology readily identified five rodenticides from complex mixtures at 1 mg L<sup>−1</sup> and one rodenticide at 10 mg L<sup>−1</sup>. Semi-quantitation was conducted through the internal standard method and negative mode ionization, with linear relationships from R2 = 0.98 to 0.99. While further optimization with alternative internal standards may be considered, this study revealed the analytical potential in DART-ToF for rapid identification and quantitation of many environmental contaminants.
dc.identifier.doi
https://doi.org/10.1039/D5AY01447F
dc.identifier.issn
1759-9679
dc.identifier.uri
https://open-science.canada.ca/handle/123456789/4230
dc.language.iso
en
dc.publisher - en
Royal Society of Chemistry
dc.publisher - fr
Royal Society of Chemistry
dc.rights - en
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.openaccesslevel - en
Gold
dc.rights.openaccesslevel - fr
Or
dc.rights.uri - en
https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr
https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en
Rodenticides
Environmental management
Detection
Poisoning
dc.subject - fr
Rodenticide
Gestion de l'environnement
Détection
Empoisonnement
dc.subject.en - en
Rodenticides
Environmental management
Detection
Poisoning
dc.subject.fr - fr
Rodenticide
Gestion de l'environnement
Détection
Empoisonnement
dc.title - en
Detection of anticoagulant rodenticides by direct analysis in real time time-of-flight mass spectrometry : novel screening techniques and rapid semi-quantitative determination
dc.type - en
Article
dc.type - fr
Article
local.article.journalissue
44
local.article.journaltitle - en
Analytical Methods
local.article.journalvolume
17
local.pagination
9000-9016
local.peerreview - en
Yes
local.peerreview - fr
Oui
local.requestdoi - en
No
local.requestdoi - fr
No
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