Salmonella enterica serovar Typhimurium temporally modulates the enteric microbiota and host responses to overcome colonization resistance in swine
Salmonella enterica serovar Typhimurium temporally modulates the enteric microbiota and host responses to overcome colonization resistance in swine
Simple item page
Full item details
- creativework.keywords - en
- swine
- colonization resistance
- salmonellosis
- temporal host responses
- microbiota responses
- creativework.keywords - fr
- porcs
- résistance à la colonisation
- salmonellose
- réponses temporelles de l'hôte
- réponses du microbiote
- dc.contributor.author
- Bescucci, Danisa M.
- Moote, Paul E.
- Polo, Rodrigo Ortega
- Uwiera, Richard R. E.
- Inglis, G. Douglas
- dc.date.accessioned
- 2023-04-04T13:24:44Z
- dc.date.available
- 2023-04-04T13:24:44Z
- dc.date.issued
- 2020-08-28
- dc.description.abstract - en
- Salmonella enterica serovar Typhimurium is a prevalent incitant of enteritis in human beings and nonhuman animals. It has been proposed that host defense responses incited by Salmonella allow the bacterium to overcome colonization resistance. Piglets (n = 24) were orally inoculated with S. enterica serovar Typhimurium DT104 or buffer alone, and the host and microbial responses were temporally examined at the acute (2 days postinoculation [dpi]), subacute (6 dpi), and recovery (10 dpi) stages of salmonellosis. At the acute stage of disease, body temperatures were elevated, and feed consumption and weight gain were reduced. The densities of Salmonella associated with the gut mucosa decreased over time, with higher densities of the bacterium in the ileum and the large intestine. Moreover, substantive histopathological changes were observed as a function of time, with prominent epithelial injury and neutrophil infiltration observed at 2 dpi. Correspondingly, a variety of host metrics were temporally affected in piglets with salmonellosis (e.g., TNFα, IFNγ, PR39, βD2, iNOS, IL8, REGIIIγ). The enteric microbiota was characterized using culture-independent and -dependent methods in concert, and taxon- and location-specific changes to the microbiota were observed in infected piglets. Bacteroides spp. (e.g., Bacteroides uniformis, Bacteroides fragilis), Streptococcus spp. (e.g., Streptococcus gallolyticus), and various Gammaproteobacteria were highly associated with inflamed tissues, while bacteria within the Ruminococcaceae and Veillonellaceae families were mainly associated with healthy mucosae. In conclusion, the study findings showed that S. Typhimurium incited temporal and spatial modifications to the swine autochthonous microbiota, and to host defense responses, that were consistent with overcoming colonization resistance to incite salmonellosis in swine.
- dc.description.plainlanguage - en
- Limited information is available on host and enteric microbiota responses incited by Salmonella enterica Typhimurium in swine, and possible mechanisms by which the bacterium overcomes colonization resistance to incite salmonellosis. Temporal characterization of a variety of host metrics in piglets (e.g. physiologic, histopathologic, and immunologic) showed the importance of studying the progression of salmonellosis. A number of host responses integrally associated with disease development were identified. Utilization of next-generation sequence analysis to characterize the enteric microbiota was found to lack sufficient resolution; however, culture-dependent and -independent methods in combination identified taxon- and location-specific changes to bacterial communities in infected piglets. The study identified bacteria and host responses associated with salmonellosis, which will be beneficial in understanding colonization resistance and for the development of effective alternatives to antibiotics to mitigate salmonellosis.
- dc.description.plainlanguage - fr
- On en connaît peu sur les réponses de l’hôte et du microbiote entérique à Salmonella enterica Typhimurium chez le porc, et sur les mécanismes possibles par lesquels la bactérie réussit à vaincre la résistance à la colonisation de l’hôte pour provoquer la salmonellose. La caractérisation temporelle de différents paramètres de l’hôte chez les porcelets (ex. physiologiques, histopathologiques et immunologiques) a montré l’importance d’étudier l’évolution de la salmonellose. Un certain nombre de réponses de l’hôte intégralement associées au développement de la maladie ont été identifiées. L’analyse des séquences de nouvelle génération n’a pas la résolution voulue pour caractériser le microbiote entérique, mais la combinaison de méthodes dépendantes et indépendantes de la culture a permis d’identifier des changements spécifiques aux taxons et à l’emplacement des communautés bactériennes chez les porcelets infectés. L’étude a permis d’identifier les bactéries et les réponses de l’hôte associées à la salmonellose, ce qui aidera à comprendre la résistance à la colonisation et à élaborer des solutions efficaces pour lutter contre la salmonellose sans avoir recours aux antibiotiques.
- dc.identifier.citation
- Bescucci, D. M., Moote, P. E., Ortega, R. O., Uwiera, R. R. E., & Inglis, G. D. (2020). Salmonella enterica serovar Typhimurium temporally modulates the enteric microbiota and host responses to overcome colonization resistance in swine. Applied and Environmental Microbiology, 86(21). https://doi.org/10.1128/AEM.01569-20
- dc.identifier.doi
- https://doi.org/10.1128/AEM.01569-20
- dc.identifier.issn
- 1098-5336
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/121
- dc.language.iso
- en
- dc.publisher
- American Society for Microbiology
- dc.rights.openaccesslevel - en
- Green
- dc.rights.openaccesslevel - fr
- Vert
- dc.subject - en
- Agriculture
- dc.subject - fr
- Agriculture
- dc.subject.en - en
- Agriculture
- dc.subject.fr - fr
- Agriculture
- dc.title - en
- Salmonella enterica serovar Typhimurium temporally modulates the enteric microbiota and host responses to overcome colonization resistance in swine
- dc.title.fosrctranslation - fr
- Salmonella enterica serovar Typhimurium temporally modulates the enteric microbiota and host responses to overcome colonization resistance in swine
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journalissue
- 21
- local.article.journaltitle
- Applied and Environmental Microbiology
- local.article.journalvolume
- 86
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
Download(s)
Original bundle
1 - 1 of 1
Name: SalmonellaEntericaSerovarTyphimuriumTemporallyModulatesTheEntericMicrobiota_2020.pdf
Size: 2.8 MB
Format: PDF
Collection(s)