Biodegradation of bovine spongiform encephalopathy prions in compost
Biodegradation of bovine spongiform encephalopathy prions in compost
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Full item details
- creativework.keywords - en
- Transmissible spongiform encephalopathies
- Prions--Pathogenicity
- Compost
- Animal watse
- creativework.keywords - fr
- Encéphalopathies spongiformes transmissibles
- Prions (Virologie)--Pathogénicité
- Compost
- Déchets animaux
- dc.contributor.author
- Xu, Shanwei
- Abeysekara, Sujeema
- Dudas, Sandor
- Czub, Stefanie
- Staskevicius, Antanas
- Mitchell, Gordon
- Amoako, Kingsley K.
- McAllister, Tim A.
- dc.date.accepted
- 2022-12-12
- dc.date.accessioned
- 2025-04-04T18:13:42Z
- dc.date.available
- 2025-04-04T18:13:42Z
- dc.date.issued
- 2022-12-23
- dc.date.submitted
- 2022-08-18
- dc.description.abstract - en
- To reduce the transmission risk of bovine spongiform encephalopathy prions (PrPBSE), specified risk materials (SRM) that can harbour PrPBSE are prevented from entering the feed and food chains. As composting is one approach to disposing of SRM, we investigated the inactivation of PrPBSE in lab-scale composters over 28 days and in bin composters over 106–120 days. Lab-scale composting was conducted using 45 kg of feedlot manure with and without chicken feathers. Based on protein misfolding cyclic amplification (PMCA), after 28 days of composting, PrPBSE seeding activity was reduced by 3–4 log10 with feathers and 3 log10 without. Bin composters were constructed using ~ 2200 kg feedlot manure and repeated in 2017 and 2018. PMCA results showed that seeding activity of PrPBSE was reduced by 1–2 log10 in the centre, but only by 1 log10 in the bottom of bin composters. Subsequent assessment by transgenic (Tgbov XV) mouse bioassay confirmed a similar reduction in PrPBSE infectivity. Enrichment for proteolytic microorganisms through the addition of feathers to compost could enhance PrPBSE degradation. In addition to temperature, other factors including varying concentrations of PrPBSE and the nature of proteolytic microbial populations may be responsible for differential degradation of PrPBSE during composting.
- dc.identifier.citation
- Xu, S., Abeysekara, S., Dudas, S., Czub, S., Staskevicius, A., Mitchell, G., Amoako, K. K., & McAllister, T. A. (2022). Biodegradation of bovine spongiform encephalopathy prions in compost. Scientific Reports, 12, Article 22233. https://doi.org/10.1038/s41598-022-26201-2
- dc.identifier.doi
- https://doi.org/10.1038/s41598-022-26201-2
- dc.identifier.issn
- 2045-2322
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3565
- dc.language.iso
- en
- dc.publisher - en
- Springer Nature
- dc.publisher - fr
- Springer Nature
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Animal diseases
- Manure
- Composting
- dc.subject - fr
- Maladie animale
- Fumier
- Compostage
- dc.subject.en - en
- Animal diseases
- Manure
- Composting
- dc.subject.fr - fr
- Maladie animale
- Fumier
- Compostage
- dc.title - en
- Biodegradation of bovine spongiform encephalopathy prions in compost
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 22233
- local.article.journaltitle - en
- Scientific Reports
- local.article.journalvolume
- 12
- local.pagination
- 1-15
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
- local.requestdoi
- No
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