Identifcation of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections
Identifcation of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections
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Full item details
- creativework.keywords - en
- Trichinellosis and other foodborne zoonotic pathogens
- Trichinella
- creativework.keywords - fr
- Trichinellose et autres pathogènes zoonotiques d'origine alimentaire
- Trichinella
- dc.contributor.author
- Lobanov, Vladislav A.
- Konecsni, Kelly A.
- Scandrett, Brad W.
- Jenkins, Emily J.
- dc.date.accepted
- 2023-10-29
- dc.date.accessioned
- 2025-03-14T18:36:38Z
- dc.date.available
- 2025-03-14T18:36:38Z
- dc.date.issued
- 2023-12-21
- dc.date.submitted
- 2023-07-06
- dc.description.abstract - en
- Amplicon-based next-generation sequencing (NGS) has rapidly gained popularity as a powerful method for delineating taxa in complex communities, including helminths. Here, we applied this approach to identify species and genotypes of zoonotic nematodes of the Trichinella genus. A known limitation of the current multiplex PCR (mPCR) assay recommended by the International Commission on Trichinellosis is that it does not differentiate Trichinella nativa from T. chanchalensis.
- dc.identifier.citation
- Lobanov, V. A., Konecsni, K. A., Scandrett, W. B., & Jenkins, E. J. (2023, December 21). Identification of trichinella taxa by its-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections - parasites & vectors. BioMed Central. https://doi.org/10.1186/s13071-023-06035-1
- dc.identifier.doi
- https://doi.org/10.1186/s13071-023-06035-1
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3524
- dc.language.iso
- en
- dc.publisher - en
- BioMed Central
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Animal diseases
- dc.subject - fr
- Maladie animale
- dc.subject.en - en
- Animal diseases
- dc.subject.fr - fr
- Maladie animale
- dc.title - en
- Identifcation of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 466 (2023)
- local.article.journaltitle - en
- Parasites & Vectors
- local.article.journalvolume
- 16
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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