SARS-CoV-2 Vaccine-Induced T-Cell Response after Three Doses in People Living with HIV on Antiretroviral Therapy Compared to Seronegative Controls (CTN 328 COVAXHIV Study)

Alexandrova, Yulia; Yero, Alexis; Bouassa,  Ralph-Sydney Mboumba; Comeau, Eve; Samarani, Suzanne; Brumme, Zabrina L.; Hull, Mark; Crawley, Angela M.; Langlois, Marc-André; Angel, Jonathan B.; Cooper, Curtis L.; Needham, Judy; Lee, Terry; Singer, Joel; Anis, Aslam H.; Costiniuk, Cecilia T.; Jenabian, Mohammad-Ali

SARS-CoV-2 Vaccine-Induced T-Cell Response after Three Doses in People Living with HIV on Antiretroviral Therapy Compared to Seronegative Controls (CTN 328 COVAXHIV Study)

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creativework.keywords - en: COVID-19; SARS-CoV-2; HIV; PLWH; vaccine; T-cell immunity; cell-mediated immunity
dc.contributor.author: Alexandrova, Yulia; Yero, Alexis; Bouassa, Ralph-Sydney Mboumba; Comeau, Eve; Samarani, Suzanne; Brumme, Zabrina L.; Hull, Mark; Crawley, Angela M.; Langlois, Marc-André; Angel, Jonathan B.; Cooper, Curtis L.; Needham, Judy; Lee, Terry; Singer, Joel; Anis, Aslam H.; Costiniuk, Cecilia T.; Jenabian, Mohammad-Ali
dc.date.accessioned: 2024-01-09T16:21:11Z
dc.date.available: 2024-01-09T16:21:11Z
dc.date.issued: 2023-02-19
dc.description.abstract - en: People living with HIV (PLWH) may be at risk for poor immunogenicity to certain vaccines, including the ability to develop immunological memory. Here, we assessed T-cell immunogenicity following three SARS-CoV-2 vaccine doses in PLWH versus uninfected controls. Blood was collected from 38 PLWH on antiretroviral therapy and 24 age-matched HIV-negative controls, pre-vaccination and after 1st/2nd/3rd dose of SARS-CoV-2 vaccines, without prior SARS-CoV-2 infection. Flow cytometry was used to assess ex vivo T-cell immunophenotypes and intracellular Tumor necrosis fac tor (TNF)-α/interferon(IFN)-γ/interleukin(IL)-2 following SARS-CoV-2-Spike-peptide stimulation. Comparisons were made using Wilcoxon signed-rank test for paired variables and Mann–Whitney for unpaired. In PLWH, Spike-specific CD4 T-cell frequencies plateaued post-2nd dose, with no significant differences in polyfunctional SARS-CoV-2-specific T-cell proportions between PLWH and uninfected controls post-3rd dose. PLWH had higher frequencies of TNFα+CD4 T-cells and lower frequencies of IFNγ+CD8 T-cells than seronegative participants post-3rd dose. Regardless of HIV status, an increase in naive, regulatory, and PD1+ T-cell frequencies was observed post-3rd dose. In summary, two doses of SARS-CoV-2 vaccine induced a robust T-cell immune response in PLWH, which was maintained after the 3rd dose, with no significant differences in polyfunctional SARS-CoV-2-specific T-cell proportions between PLWH and uninfected controls post-3rd dose.
dc.identifier.citation: Alexandrova Y, Yero A, Mboumba Bouassa RS, Comeau E, Samarani S, Brumme ZL, Hull M, Crawley AM, Langlois MA, Angel JB, Cooper CL, Needham J, Lee T, Singer J, Anis AH, Costiniuk CT, Jenabian MA. SARS-CoV-2 Vaccine-Induced T-Cell Response after Three Doses in People Living with HIV on Antiretroviral Therapy Compared to Seronegative Controls (CTN 328 COVAXHIV Study). Viruses. 2023 Feb 19;15(2):575. doi: 10.3390/v15020575
dc.identifier.doi: https://doi.org/ 10.3390/v15020575
dc.identifier.issn: 1999-4915
dc.identifier.pubmedID: 36851789
dc.identifier.uri: https://open-science.canada.ca/handle/123456789/1605
dc.language.iso: en
dc.publisher: MDPI
dc.rights - en: Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr: Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.openaccesslevel - en: Gold
dc.rights.openaccesslevel - fr: Or
dc.rights.uri - en: https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr: https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en: Health
dc.subject - fr: Santé
dc.subject.en - en: Health
dc.subject.fr - fr: Santé
dc.title - en: SARS-CoV-2 Vaccine-Induced T-Cell Response after Three Doses in People Living with HIV on Antiretroviral Therapy Compared to Seronegative Controls (CTN 328 COVAXHIV Study)
dc.type - en: Article
dc.type - fr: Article
local.acceptedmanuscript.articlenum: 575
local.article.journalissue: 2
local.article.journaltitle: Viruses
local.article.journalvolume: 15
local.peerreview - en: Yes
local.peerreview - fr: Oui