Two independent approaches converge to the cloning of a new Leptosphaeria maculans avirulence effector gene, AvrLmS-Lep2

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dc.contributor.author

Xiang Neik, Ting

Ghanbarnia, Kaveh

Ollivier, Bénédicte

Scheben, Armin

Severn-Ellis, Anita

Larkan, Nicholas J.

Haddadi, Parham

Fernando, Dilantha W. G.

Rouxel, Thierry

Batley, Jacqueline

Borhan, Hossein M.

Balesdent, Marie-Hélène

dc.date.accepted

2022-02-01

dc.date.accessioned

2024-02-27T16:36:12Z

dc.date.available

2024-02-27T16:36:12Z

dc.date.issued

2022-03-22

dc.date.submitted

2021-07-12

dc.description.abstract - en

Brassica napus (oilseed rape, canola) seedling resistance to Leptosphaeria maculans, the causal agent of blackleg (stem canker) disease, follows a gene-for-gene relationship. The avirulence genes AvrLmS and AvrLep2 were described to be perceived by the resistance genes RlmS and LepR2, respectively, present in B. napus ‘Surpass 400’. Here we report cloning of AvrLmS and AvrLep2 using two independent methods. AvrLmS was cloned using combined in vitro crossing between avirulent and virulent isolates with sequencing of DNA bulks from avirulent or virulent progeny (bulked segregant sequencing). AvrLep2 was cloned using a biparental cross of avirulent and virulent L. maculans isolates and a classical map-based cloning approach. Taking these two approaches independently, we found that AvrLmS and AvrLep2 are the same gene. Complementation of virulent isolates with this gene confirmed its role in inducing resistance on Surpass 400, Topas-LepR2, and an RlmS-line. The gene, renamed AvrLmS-Lep2, encodes a small cysteine-rich protein of unknown function with an N-terminal secretory signal peptide, which is a common feature of the majority of effectors from extracellular fungal plant pathogens. The AvrLmS-Lep2/LepR2 interaction phenotype was found to vary from a typical hypersensitive response through intermediate resistance sometimes towards susceptibility, depending on the inoculation conditions. AvrLmS-Lep2 was nevertheless sufficient to significantly slow the systemic growth of the pathogen and reduce the stem lesion size on plant genotypes with LepR2, indicating the potential efficiency of this resistance to control the disease in the field.

dc.identifier.citation

Xiang, Neik T., Ghanbarnia, K., Ollivier, B., Scheben, A., Severn-Ellis, A., Larkan, N.J., Haddadi, P., Fernando, D. W. G., Rouxel, T., Batley, J., Borhan, H.M., & Balesdent, M.-H. (2022). Two independent approaches converge to the cloning of a new Leptosphaeria maculans avirulence effector gene, AvrLmS-Lep2. Molecular Plant Pathology, 23(5), 733–748. https://doi.org/10.1111/mpp.13194

dc.identifier.doi

https://doi.org/10.1111/mpp.13194

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/1974

dc.language.iso

en

dc.publisher

John Wiley and Sons Inc

dc.rights - en

Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

dc.rights - fr

Creative Commons Attribution-Pas d’Utilisation Commerciale 4.0 International (CC BY-NC 4.0)

dc.rights.openaccesslevel - en

Gold

dc.rights.openaccesslevel - fr

Or

dc.rights.uri - en

https://creativecommons.org/licenses/by-nc/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by-nc/4.0/deed.fr

dc.subject - en

Agriculture

dc.subject - fr

Agriculture

dc.subject.en - en

Agriculture

dc.subject.fr - fr

Agriculture

dc.title - en

Two independent approaches converge to the cloning of a new Leptosphaeria maculans avirulence effector gene, AvrLmS-Lep2

dc.type - en

Article

dc.type - fr

Article

local.article.journalissue

5

local.article.journaltitle

Molecular Plant Pathology

local.article.journalvolume

23

local.pagination

733-748

local.peerreview - en

Yes

local.peerreview - fr

Oui

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