Canola meal valorization via acid hydrolysis to generate free amino acids
Canola meal valorization via acid hydrolysis to generate free amino acids
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Full item details
- creativework.keywords - en
- canola meal
- proteins
- ethanol pre-treatment
- free amino acids
- response surface methodology
- hydrology
- creativework.keywords - fr
- tourteau de colza canola
- prétraitement à l'ethanol
- protéines
- acides aminés libre
- méthode de surface de réponse
- hydrolyse
- dc.contributor.author
- Warnakulasuriya, Sumudu N.
- Tanaka, Takuji
- Wanasundara, Janitha P. D.
- dc.date.accepted
- 2023-08-11
- dc.date.accessioned
- 2024-07-30T21:37:03Z
- dc.date.available
- 2024-07-30T21:37:03Z
- dc.date.issued
- 2023-09-22
- dc.date.submitted
- 2023-06-06
- dc.description.abstract - en
- This study investigated an alternative approach to valorizing canola proteins by hydrolyzing them to generate amino acids (AAs). Pre-treatment of cold-pressed (CP) cake and desolventized-toasted (DT) meal with ethanol (99%, vol/vol) followed by protein separation was studied as process optimizations to maximize protein recovery with higher purity. The optimum ethanol pre-treatment conditions to achieve a meal containing less than 1% oil was reached at a meal-to-ethanol ratio of 1:4 (wt:wt) and 50°C for 30 min extraction. The protein recovery reached the maximum at pH 12 and a meal-to-solvent ratio of 1:10 (wt:vol), yielding 73% and 33% recovery from ethanol pre-treated CP and DT meals, respectively, in a single extraction. Untreated and ethanol pre-treated meals were hydrolyzed with 6 N HCl (protein-to-acid ratio of 5 mg:2 mL) for 24 h at 110°C. The ethanol pre-treatment improved AA recovery and released 373 mg AA/g dry CP meal biomass (dbm) compared to 279 mg AA/g untreated CP cake dbm. However, no improvement in AA recovery upon ethanol pre-treatment of DT meal. Sulfuric acid was examined as an alternative acid. More than 700 mg AA/g CP protein were released with 6 N H2SO4, while for DT meal proteins, a 10 N concentration was needed to achieve a closer value. Commercial canola meals can be utilized for generating free AAs; however, the meal processing history may affect the yield.
- dc.identifier.citation
- Warnakuluasuriya, S. N., Tanaka, T., & Wanasundara, J. P. D. (2024). Canola meal valorization via acid hydrolysis to generate free amino acids. Journal of the American Oil Chemists' Society, 101, 41-57. https://doi.org/10.1002/aocs.12739
- dc.identifier.doi
- https://doi.org/10.1002/aocs.12739
- dc.identifier.issn
- 1558-9331
- 0003-021X
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/2772
- dc.language.iso
- en
- dc.publisher
- Wiley Periodicals LLC, AOCS
- dc.rights - en
- Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
- dc.rights - fr
- Creative Commons Attribution - Pas d'utilisation commerciale - Pas de modification 4.0 International (CC BY-NC-ND 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by-nc-nd/4.0/deed.fr
- dc.subject - en
- Agriculture
- Science and technology
- dc.subject - fr
- Agriculture
- Sciences et technologie
- dc.subject.en - en
- Agriculture
- Science and technology
- dc.subject.fr - fr
- Agriculture
- Sciences et technologie
- dc.title - en
- Canola meal valorization via acid hydrolysis to generate free amino acids
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journaltitle
- Journal of the American Oil Chemists' Society
- local.article.journalvolume
- 101
- local.pagination
- 41-57
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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