Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody

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creativework.keywords - en

Serodiagnosis

Zoonotic and emerging pathogens

Zoonosis

creativework.keywords - fr

Sérodiagnostics

Pathogènes zoonotiques et émergents

Zoonoses

dc.contributor.author

Zhu, Wenjun

Pickering, Bradley

Smith, Greg

Pinette, Mathieu

Truong, Thang

Babiuk, Shawn

Kobasa, Darwyn

Banadyga, Logan

Yang, Ming

dc.date.accepted

2023-01-13

dc.date.accessioned

2024-08-29T13:23:19Z

dc.date.available

2024-08-29T13:23:19Z

dc.date.issued

2023-02-01

dc.date.submitted

2022-12-16

dc.description.abstract - en

Introduction: Nipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality. Methods: A competitive enzyme–linked immunosorbent assay (cELISA) using a monoclonal antibody (mAb) and recombinant NiV glycoprotein (G) was developed and laboratory evaluated using sera from experimental pigs, mini pigs and nonhuman primates. The test depends on competition between specific antibodies in positive sera and a virus–specific mAb for binding to NiV–G. Results: Based on 1,199 negative and 71 NiV positive serum test results, the cutoff value was determined as 35% inhibition. The diagnostic sensitivity and specificity of the NiV cELISA was 98.58 and 99.92%, respectively. When testing sera from animals experimentally infected with NiV Malaysia, the cELISA detected antibodies from 14 days post–infection (dpi) and remained positive until the end of the experiment (28 dpi). Comparisons using the Kappa coefficient showed strong agreement (100%) between the cELISA and a plaque reduction neutralization test (PRNT). Discussion: Because our cELISA is simpler, faster, and gives comparable or better results than PRNT, it would be an adequate screening test for suspect NiV and HeV cases, and it would also be useful for epidemiological surveillance of Henipavirus infections in different animal species without changing reagents.

dc.identifier.citation

Zhu, W., Pickering, B., Smith, G., Pinette, M., Truong, T., Babiuk, S., Kobasa, D., Banadyga, L., & Yang, M. (2023). Development and laboratory evaluation of a competitive Elisa for serodiagnosis of Nipah and hendra virus infection using recombinant nipah glycoproteins and a monoclonal antibody. Frontiers in Veterinary Science, 10. https://doi.org/10.3389/fvets.2023.1120367

dc.identifier.doi

https://doi.org/10.3389/fvets.2023.1120367

dc.identifier.uri

https://open-science.canada.ca/handle/123456789/2898

dc.language.iso

en

dc.publisher

Frontiers Media S.A.

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.openaccesslevel - en

Gold

dc.rights.openaccesslevel - fr

Or

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Agriculture

Health and safety

dc.subject - fr

Agriculture

Santé et sécurité

dc.subject.en - en

Agriculture

Health and safety

dc.subject.fr - fr

Agriculture

Santé et sécurité

dc.title - en

Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody

dc.type - en

Article

dc.type - fr

Article

local.article.journaltitle

Frontiers in Veterinary Science

local.article.journalvolume

10

local.peerreview - en

Yes

local.peerreview - fr

Oui

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