Molecular characterization and comparisons of potato wart (Synchytrium endobioticum) in historic collections to recent findings in Canada and the Netherlands

van de Vossenberg, Bart T.L.H.; van Gent, Marga; Meffert, Johan P.; Nguyen, Hai D.T.; Smith, Donna; van Kempen, Thijn; Helderman, Carin M.; Rosendahl-Peters, Karin C.H.M.; Braak, Naomi te; Flath, Kerstin; Przetakiewicz, Jarosław; Perez, Willmer; Çakir, Emel; Sikharulidze, Zoia V.; van Leeuwen, Gerard C.M.; van der Lee, Theo A.J.

doi:https://open-science.canada.ca/handle/123456789/2851

Molecular characterization and comparisons of potato wart (Synchytrium endobioticum) in historic collections to recent findings in Canada and the Netherlands

Simple item page

Full item details

creativework.keywords - en: Potato wart; Galle verruqueuse de la pomme de terre
creativework.keywords - fr: Pomme de terre--Maladies et fléaux; Potatoes--Dieseases and pests
dc.contributor.author: van de Vossenberg, Bart T.L.H.; van Gent, Marga; Meffert, Johan P.; Nguyen, Hai D.T.; Smith, Donna; van Kempen, Thijn; Helderman, Carin M.; Rosendahl-Peters, Karin C.H.M.; Braak, Naomi te; Flath, Kerstin; Przetakiewicz, Jarosław; Perez, Willmer; Çakir, Emel; Sikharulidze, Zoia V.; van Leeuwen, Gerard C.M.; van der Lee, Theo A.J.
dc.date.accepted: 2022-12-16
dc.date.accessioned: 2024-08-19T14:13:47Z
dc.date.available: 2024-08-19T14:13:47Z
dc.date.issued: 2023-01-02
dc.date.submitted: 2022-08-26
dc.description.abstract - en: Synchytrium endobioticum (Schilb.) Perc. is a chytrid fungus causing potato wart disease and is one of the most important quarantine diseases on cultivated potato. Infected host tissues develop warts rendering the crop unmarketable. Resting spores, that can remain viable and infectious for decades, are formed in warted tissues and are released into the surrounding soil when host tissue decays. To better understand the pathogen’s diversity and to potentially uncover pathways of migrations and introduction events, molecular characterization was performed on the historical S. endobioticum resting spore collection of the Dutch National Plant Protection Organization. Mitochondrial genomes were assembled and annotated, and four novel structural variants were identified from these materials with intronic presence-absence variation in cox1 or cob genes and structural variation in the dpoB – TIR region. Several fungal isolates were shown to contain mixtures of structural variants. We analyzed the mitogenomic sequences obtained from recent potato wart disease findings in Canada and the Netherlands in the context of the historical materials and found that fungal isolates from the new Dutch outbreak contained a specific mixture of mitogenomic variants previously not observed in the Netherlands. Based on the mitogenomic profile, pathotype 38(Nevşehir) was suspected which was later verified with the Spieckermann bioassay. To further facilitate dissemination of data and interactive visual analytics we created a public Nextstrain webpage with S. endobioticum mitogenomic sequences and associated metadata on their geographic origin, pathotype identity and (mixture) of mitogenomic variants (https://nextstrain.nrcnvwa.nl/Sendo).
dc.description.fosrcfull - en: Published online in 2023. Published in print in 2024.
dc.description.fosrcfull-fosrctranslation - fr: Publié en ligne en 2023. Publié en version imprimée en 2024.
dc.identifier.citation: van de Vossenberg, B. T. L. H., van Gent, M., Meffert, J. P., Nguyen, H. D. T., Smith, D., van Kempen, T., Helderman, C. M., Rosendahl-Peters, K. C. H. M., Braak, N. te, Flath, K., Przetakiewicz, J., Perez, W., Çakir, E., Sikharulidze, Z. V., van Leeuwen, G. C. M., & van der Lee, T. A. J. (2024). Molecular characterization and comparisons of potato wart (Synchytrium endobioticum) in historic collections to recent findings in Canada and the Netherlands. Journal of Plant Pathology, 106(2), 363-375. https://doi.org/10.1007/s42161-022-01299-5
dc.identifier.doi: https://doi.org/10.1007/s42161-022-01299-5
dc.identifier.issn: 2239-7264; 1125-4653
dc.identifier.uri: https://open-science.canada.ca/handle/123456789/2851
dc.language.iso: en
dc.publisher: Springer Science and Business Media Deutschland GmbH
dc.rights - en: Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights - fr: Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.rights.openaccesslevel - en: Gold
dc.rights.openaccesslevel - fr: Or
dc.rights.uri - en: https://creativecommons.org/licenses/by/4.0/
dc.rights.uri - fr: https://creativecommons.org/licenses/by/4.0/deed.fr
dc.subject - en: Agriculture
dc.subject - fr: Agriculture
dc.subject.en - en: Agriculture
dc.subject.fr - fr: Agriculture
dc.title - en: Molecular characterization and comparisons of potato wart (Synchytrium endobioticum) in historic collections to recent findings in Canada and the Netherlands
dc.type - en: Article
dc.type - fr: Article
local.article.journalissue: 2
local.article.journaltitle: Journal of Plant Pathology
local.article.journalvolume: 106
local.pagination: 363-375
local.peerreview - en: Yes
local.peerreview - fr: Oui
local.requestdoi: No

Download(s)

Original bundle

Now showing 1 - 1 of 1

Name: MolecularCharacterizationAndComparisonsOfPotatoWartInHistoricCollectionsToRecentFindingsInCanadaAndTheNetherlands_2023.pdf

Size: 5.31 MB

Format: PDF

Download file

Collection(s)

Plants