Spleen swabs for sensitive and high-throughput detection of African swine fever virus by real-time PCR

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DOI

https://doi.org/10.3390/v16081316

Langue de publication
Anglais
Date
2024-08-18
Type
Article
Auteur(s)
  • Cafariello, Christopher
  • Goonewardene, Kalhari
  • Chung, Chungwon J.
  • Ambagala, Aruna
Éditeur
MDPI

Résumé

African swine fever (ASF) continues to spread in Africa, Europe, Asia and the island of Hispaniola, increasing the need to develop more streamlined and highly efficient surveillance and diagnostic capabilities. One way to achieve this is by further optimization of already established standard operating procedures to remove bottlenecks for high-throughput screening. Real-time polymerase chain reaction (real-time PCR) is the most sensitive and specific assay available for the early detection of the ASF virus (ASFV) genome, but it requires high-quality nucleic acid extracted from the samples. Whole blood from live pigs and spleen tissue from dead pigs are the preferred samples for real-time PCR. Whole blood can be used as is in nucleic acid extractions, but spleen tissues require an additional homogenization step. In this study, we compared the homogenates and swabs prepared from 52 spleen samples collected from pigs experimentally inoculated with highly and moderately virulent ASF virus strains. The results show that not only are the spleen swabs more sensitive when executed with a low-cell-count nucleic acid extraction procedure followed by real-time PCR assays but they also increase the ability to isolate ASFV from positive spleen samples. Swabbing is a convenient, simpler and less time-consuming alternative to tissue homogenization. Hence, we recommend spleen swabs over tissue homogenates for high-throughput detection of ASFV by real-time PCR.

Sujet

  • Sciences et technologie,
  • Virus

Mots-clés

  • African and classical swine fever,
  • ASF,
  • CSF

Droits

Creative Commons Attribution 4.0 International (CC BY 4.0)

Évalué par les pairs

Yes

Identifiants

ISSN
1999-4915

Article

Titre de la revue
Virus
Volume de la revue
16
Numéro de revue
8
Numéro de l'élément
1316
Date d'acceptation
2024-08-16
Date de soumission
2024-07-25

Référence(s)

Cafariello, C., Goonewardene, K., Chung, C. J., & Ambagala, A. (2024). Spleen swabs for sensitive and high-throughput detection of African swine fever virus by real-time PCR. Viruses, 16(8), 1316. https://doi.org/10.3390/v16081316

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Animals

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