Salmonid smolt caudal fin and liver transcriptome responses to low sulfur marine diesel and high sulfur fuel oil water accommodated fractions for assessing oil spill effects in marine environments
Salmonid smolt caudal fin and liver transcriptome responses to low sulfur marine diesel and high sulfur fuel oil water accommodated fractions for assessing oil spill effects in marine environments
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Full item details
- dc.contributor.author
- Imbery, Jacob J.
- Buday, Craig
- Miliano, Rachel C.
- Shang, Dayue
- Kwok, Honoria
- Helbing, Caren C.
- dc.date.accepted
- 2024-05-12
- dc.date.accessioned
- 2026-02-10T16:31:44Z
- dc.date.available
- 2026-02-10T16:31:44Z
- dc.date.issued
- 2024-05-15
- dc.date.submitted
- 2024-01-21
- dc.description - en
- Exposure to oil product seawater accommodated fractions (seaWAF) can be deleterious. There is a need for effectively tracking seaWAF exposure effects in salmon. Coho salmon smolt were exposed to marine diesel and high sulfur fuel oil seaWAF. RNA-sequencing (RNA-seq) and quantitative polymerase chain reaction (qPCR) indicated caudal fin and liver candidate transcript profiles. Caudal fin, a nonlethal liver-alternative, is highly responsive to seaWAF exposure.
- dc.description.abstract - en
- The environmental impact of oil spills is a critical concern, particularly pertaining to low sulfur marine diesel (LSMD) and high sulfur fuel oil (HSFO) that are commonly involved in coastal spills. Although transcriptomic biomonitoring of sentinel animals can be a powerful tool for assessing biological effects, conventional methods utilize lethal sampling to examine the liver. As a non-lethal alternative, we have previously shown salmonid caudal fin cyp1a1 is significantly responsive to LSMD-derived toxicants. The present study further investigated the transcriptomic biomonitoring potential of coho salmon smolt caudal fin in comparison to liver tissue in the context of LSMD and HSFO seawater accommodated fraction (seaWAF) exposure in cold-water marine environments. Assessing the toxicity of these seaWAFs involved quantifying polycyclic aromatic hydrocarbon (tPAH50) concentrations and generating gene expression profiles. Initial qPCR analyses revealed significant cyp1a1 response in both liver and caudal fin tissues of both genetic sexes to all seaWAF exposures. RNA-Seq analysis, focusing on the highest LSMD and HSFO seaWAF concentrations (28.4±1.8 and 645.08±146.3 µg/L tPAH50, respectively), revealed distinct tissue-specific and genetic sex-independent transcriptomic responses with an overall enrichment of oxidative stress, cell adhesion, and morphogenesis-related pathways. Remarkably, the caudal fin tissue exhibited transcriptomic response patterns comparable to liver tissue, particularly consistent differential expression of 33 gene transcripts in the liver (independent of sex and oil type) and 44 in the caudal fin. The present work underscores the viability of using the caudal fin as a non-lethal alternative to liver sampling for assessing and tracking oil spill exposure in marine environments.
- dc.description.fosrctranslation - fr
- L’exposition à des fractions adaptées à l’eau de mer (seaWAF) peut être délétère. Il est nécessaire d’assurer un suivi efficace des effets de l’exposition des saumons aux seaWAF. Des smolts de cohos ont été exposés aux seaWAF de combustibles de soute et de mazout à haute teneur en soufre. Le séquençage de l’ARN et l’amplification en chaîne par polymérase quantitative (qPCR) ont révélé des profils de transcrits candidats pour la nageoire caudale et le foie. La nageoire caudale, un substitut au foie constituant une méthode non létale, réagit fortement à l’exposition aux seaWAF.
- dc.identifier.doi
- https://doi.org/10.1016/j.ecoenv.2024.116463
- dc.identifier.issn
- 1090-2414
- 0147-6513
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/4234
- dc.language.iso
- en
- dc.publisher - en
- Elsevier
- dc.publisher - fr
- Elsevier
- dc.rights - en
- Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
- dc.rights - fr
- Creative Commons Attribution-Pas d’Utilisation Commerciale 4.0 International (CC BY-NC 4.0)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/licenses/by-nc/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by-nc/4.0/deed.fr
- dc.subject - en
- Oil spills
- Salt water fish
- Petroleum
- dc.subject - fr
- Déversement de pétrole
- Poisson de mer
- Pétrole
- dc.subject.en - en
- Oil spills
- Salt water fish
- Petroleum
- dc.subject.fr - fr
- Déversement de pétrole
- Poisson de mer
- Pétrole
- dc.title - en
- Salmonid smolt caudal fin and liver transcriptome responses to low sulfur marine diesel and high sulfur fuel oil water accommodated fractions for assessing oil spill effects in marine environments
- dc.title.alternative - fr
- Réponses du transcriptome de la nageoire caudale et du foie de smolts aux fractions adaptées à l’eau de combustibles de soute à faible teneur en soufre et de mazout à haute teneur en soufre aux fins d’évaluation des effets des déversements d’hydrocarbures dans les milieux marins
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 116463
- local.article.journaltitle - en
- Ecotoxicology and Environmental Safety
- local.article.journalvolume
- 279
- local.pagination
- 10 pages
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
- local.requestdoi - en
- No
- local.requestdoi - fr
- No
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