Research Article Integrated InVivoGenotoxicityAssessment of Procarbazine HydrochlorideDemonstrates InductionofPig-a andLacZMutations, andMicronuclei, inMutaMouseHematopoietic Cells ClotildeMaurice,1StephenD.Dertinger,2 Carole L.Yauk,1and FrancescoMarchetti 1* 1Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada 2Litron Laboratories, Rochester, New York Procarbazine hydrochloride (PCH) is a DNA-reactive hematopoietic carcinogen with potent and well- characterized clastogenic activity. However, there is a paucity of in vivo mutagenesis data for PCH, and in vitro assays often fail to detect the genotoxic effects of PCH due to the complexity of its metabolic activation. We comprehensively evaluated the in vivo genotoxicity of PCH on hematopoietic cells of male MutaMouse transgenic rodents using a study design that facilitated assessments of micronu- clei and Pig-a mutation in circulating erythrocytes, and lacZ mutant frequencies in bone marrow. Mice were orally exposed to PCH (0, 6.25, 12.5, and 25 mg/kg/day) for 28 consecutive days. Blood samples collected 2 days after cessation of treat- ment exhibited significant dose-related induction of micronuclei in both immature and mature erythro- cytes. Bone marrow and blood collected 3 and 70 days after cessation of treatment also showed significantly elevated mutant frequencies in both the lacZ and Pig-a assays even at the lowest dose tested. PCH-induced lacZ and Pig-a (immature and mature erythrocytes) mutant frequencies were highly correlated, with R2 values ≥0.956, with the excep- tion of lacZ vs. Pig-a mutants in mature erythrocytes at the 70-day time point (R2 = 0.902). These results show that PCH is genotoxic in vivo and demonstrate that the complex metabolism and resulting genotoxi- city of PCH is best evaluated in intact animal models. Our results further support the concept that multiple biomarkers of genotoxicity, especially hematopoietic cell genotoxicity, can be readily combined into one study provided that adequate attention is given to manifestation times. Environ. Mol. Mutagen. 60:505–512, 2019. © 2018 Her Majesty the Queen in Right of Canada Key words:mutagenicity; bonemarrow; red blood cells;mutation;micronuclei INTRODUCTION Procarbazine hydrochloride (PCH) is a hydrazine deriva- tive that is reemerging as a useful drug that in combination with other anti-neoplastic agents is effective at treating several malignancies, especially gliomas and Hodgkin’s lymphoma (Armand et al. 2007). While clearly efficacious, concerns about PCH treatment-induced tumors exist (Levine and Bloomfield 1992). Mouse bioassays indicate that lung and hematopoietic cells are important target sites (IARC 1981). Furthermore, cancer patients treated with PCH in combination with other antineoplastic agents have an elevated risk of secondary malignancies, especially hematopoietic cancers (IARC 1981; Kaldor et al. 1988). PCH appears to be a particularly effective in vivo clasto- gen inducing both micronuclei in bone marrow (Romagna and Schneider 1990) and DNA breaks as detected by the alkaline elution (Holme et al. 1989) and comet (Sasaki et al. 1998) assays in multiple tissues including stomach and liver. Furthermore, significant increases in median percent tail DNA were recently reported in bone marrow, liver, kidney, and lungs of rats, 1 day after a 28-day expo- sure to 30 or 60 mg PCH/kg/day (Chen et al. 2019). How- ever, PCH’s mutagenic activity is less well-characterized. For instance, using the transgenic MutaMouse model, Suzuki et al. (1999) showed that a single intraperitoneal (i.p.) injection of 50 mg PCH/kg was a potent micronucleus Additional Supporting Information may be found in the online version of this article. Reproduced with the permission of the Minister of Environmental Health Science and Research Bureau Grant sponsor: Natural Sciences and Engineering Research Council of Canada. Grant sponsor: Health Canada. *Correspondence to: Francesco Marchetti, Environmental Health Science Research Bureau, Health Canada, 50 Colombine Driveway, Ottawa, Ontario, K1A 0K9, Canada. E-mail: francesco.marchetti@canada.ca Received 30 October 2018; provisionally accepted 21 December 2018; and in final form 26 December 2018 DOI: 10.1002/em.22271 Published online 28 December 2018 in Wiley Online Library (wileyonlinelibrary.com). Environmental andMolecularMutagenesis 60:505^512 (2019) © 2018 TheAuthors.Environmental andMolecularMutagenesis published by Wiley Periodicals, Inc. on behalf of EnvironmentalMutagen Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://orcid.org/0000-0002-9435-4867 mailto:francesco.marchetti@canada.ca http://creativecommons.org/licenses/by/4.0/ inducer in blood (mean fold change: 19.6x), while no change to lacZ mutation frequencies were observed in mul- tiple tissues. In contrast, PCH mutagenicity was evident in lung, bone marrow, and spleen after five daily administrations of 150 mg/kg by i.p. injection (Suzuki et al. 1999). Following a 3-day exposure regimen by oral gavage Phonethepswath et al. (2013) confirmed the strong clastogenic activity of PCH and observed a moderate but variable mutagenic response using the rodent phosphatidylinositol glycan-class A (Pig-a) assay. Together with the rodent bioassay data and occurrences of therapy-related tumors noted above, the weight of evidence provided by in vivo data implies that PCH is a carcinogen with a genotoxic mode of action that clearly involves clasto- genicity, with some evidence of mutagenicity as well. Based on these findings, the mutagenic potential of PCH deserves more analysis and consideration, including an assessment of the impact of longer exposure duration, different sampling times, and a comparison of different genetic endpoints within the same experimental system. PCH undergoes complex metabolic activation and forms intermediates that have the capacity to react with DNA. One presumably important intermediate is the methyl dia- zonium ion that induces O6-methyl-guanine adducts (Souliotis et al. 1994; Pletsa et al. 1997). The complexity of PCH metabolism is illustrated by the fact that a free rad- ical, a reactive aldehyde, and an arylmethyl diazonium ion have also been implicated as significant contributors to observed toxic effects (IARC 1981; Prough and Tweedie 1987; Suzuki et al. 1999). Given the reactivity of these var- ious metabolites, the well-documented in vivo genotoxicity of the drug is not surprising (IARC 1981). At the same time, the complex metabolism of PCH coupled with defi- ciencies with standard exogenous metabolic activation sys- tems is considered responsible for the high occurrence of false negative in vitro results (reviewed in Suzuki et al. 1999). Indeed, bacterial mutagenicity data are gener- ally negative (McCann et al. 1975; Bronzetti et al. 1979). Mutagenic effects were observed in V79 cells (Suter 1987), but the clearest effects required nonstandard metabolic acti- vation systems. There are some reports of positive mouse lymphoma assay data with the resultant colonies being described as small, suggesting a clastogenic mode of action (Clive et al. 1988). However, Vian et al. (1993) failed to induce micronuclei in cultures of PCH-treated primary human lymphocytes, with or without rat liver S9. Thus, PCH represents an example where the genotoxic potential is best evaluated in vivo. There is growing interest in integrating various testing approaches to significantly improve the manner in which genotoxicity testing is accomplished and reducing the num- ber of animals required for testing. These integrated approaches provide a mechanism to precisely quantify dif- ferent genotoxic endpoints within the same experimental design and identify which endpoints are most relevant to adverse effects in humans. We therefore considered PCH to be an interesting test case for evaluating the merits of an integrated study design that would allow us to comprehen- sively assess hematopoietic cell genotoxicity, both for chromosomal damage and gene mutation potential. The treatment period consisted of 28 consecutive days of exposure, and evaluation of chromosomal damage was accomplished by measuring micronucleus frequencies in both immature and mature peripheral blood erythrocytes (MacGregor et al. 1980). We used the MutaMouse trans- genic mouse model that harbor ~29 tandem copies of a recombinant λgt10 phage vector containing a mutation- reporting Escherichia coli lacZ gene on each copy of chro- mosome 3 (Shwed et al. 2010) and facilitated assessments of mutations in both the lacZ transgene (Gingerich et al. 2014) and the endogenous Pig-a gene (Gollapudi et al. 2015). We quantified mutations arising in these two reporter genes at two time points: (1) 3 days from the end of the last treatment as recommended in the Organisation of Economic Co-operation and Development (OECD) test guideline 488 (OECD 2013); and (2) 70 days after cessa- tion of treatment to evaluate effects in long-lived progenitor cells. The results are discussed in terms of the high infor- mation content provided by this multiple endpoint study design, including comparison of mutation frequencies at two gene loci, one endogenous and one transgenic, and chromosomal damage. MATERIALS ANDMETHODS Reagents andOther Supplies PCH (CAS no. 36670-1; purity ≥98%) was purchased from Sigma- Aldrich, Oakville, ON, Canada. Lympholyte®-Mammal cell separation reagent was purchased from CedarLane, Burlington, NC. Anti-PE MicroBeads, LS Columns, and a QuadroMACS™ separator were from Miltenyi Biotec, Bergisch Gladbach, Germany. CountBright™ absolute count beads and fetal bovine serum (FBS) were purchased from Invitro- gen, Carlsbad, CA. Anticoagulant solution, buffered salt solution, nucleic acid dye solution (contains SYTO® 13), anti-CD24-PE, and anti-CD61-PE were from Mouse Blood In Vivo MutaFlow® Kits (Litron Laboratories, Rochester, NY). Hank’s balanced salt solution (HBSS) was purchased from MediaTech, Herndon, VA. Reagents used for flow cytometric micro- nucleus scoring (anticoagulant solution, buffered salt solution, stock propi- dium iodide solution, anti-CD71-FITC, and anti-CD61-PE solutions, stock RNase solution, and malaria biostandards) were from In Vivo Mouse MicroFlow® Kits (Litron Laboratories). For the lacZ assay, phenyl-beta-D-galactopyranoside (P-Gal) was purchased from G-Biosciences, St Louis, MO; proteinase K was from Invitrogen, Burlington, ON, Canada; packaging extract kits were from Agilent Technologies, Mississauga, ON, Canada; all other chemicals were purchased from Sigma-Aldrich. Animals,Treatments, BloodHarvests Experiments were conducted with the oversight of the Health Canada Ottawa Animal Care Committee, which approved all animal procedures. Male MutaMouse rodents were obtained from a colony maintained at Health Canada. Mice were allowed to acclimate for approximately 10 days, and their age at the start of the treatment was between 10 and 12 weeks. Environmental and Molecular Mutagenesis. DOI 10.1002/em 506 Maurice et al. Pretreatment blood samples were not collected prior to study initiation. Water and food were available ad libitum throughout the acclimation and experimental periods. PCH was prepared in phosphate buffered saline (PBS) on each treatment day and was administered via oral gavage in a volume of 0.003 mL/kg body weight/administration. Dose levels were 0, 6.25, 12.5, or 25 mg/kg/day and exposure occurred on study Days 1 through 28 (n = 4 for controls and 8 per treatment group per time point). The top dose level used in the main study was based on a pilot dose range-finding study that demonstrated that a dose of 50 mg/kg/day resulted in a body weight loss that approached what would be considered excessive toxicity and grossly affected testis weight (this aspect will be reported sep- arately, manuscript in progress). For the micronucleus assay, peripheral blood was collected 2 days after the end of the exposure period (referred to hereafter as 28 + 2d) from the same groups of animals euthanized the next day (i.e., 28 + 3d) for the lacZ and Pig-a assays. Approximately 60 μL of peripheral blood was collec- ted from the facial vein and immediately combined with 350 μL of antico- agulant from the MicroFlowBASIC Kit (Rodent Fixed Blood; Litron Laboratories, Rochester, NY). Following MicroFlowBASIC Kit instructions, anticoagulated blood samples were fixed with ice-cold methanol and stored at −80�C. After 5 days, fixed blood samples were centrifuged, rinsed, and transferred to a long-term storage solution. Coded specimens were shipped to Litron for analysis of micronucleated cell frequency as described below. Peripheral blood and bone marrow for the Pig-a and lacZ assays, respectively, were collected at 28 + 3d and 70 days (28 + 70d) after cessa- tion of treatment. At each time point, animals were euthanized by cardiac puncture under isoflurane anesthesia. The femurs were removed and the bone marrow was flushed out in 1 mL of PBS, pelleted via brief centri- fugation, resuspended in 100 μL of PBS and flash frozen in liquid nitrogen, and finally stored at −80�C. Following blood collection via car- diac puncture, 400 μL of blood was immediately transferred to a 0.5 mL K2-EDTA-coated microtainer obtained from Litron and maintained at 4�C until being placed in an ExactPak shipping container and shipped on ice overnight to Litron for analysis of Pig-a mutant cell frequencies as described below. Micronucleated Reticulocytes: Sample Preparation,Data Acquisition The frequency of reticulocytes (%RET), micronucleated immature, and mature (normochromatic) erythrocytes (MN-RET and MN-NCE, respec- tively) were determined for coded blood samples collected at the 28 + 2d time point. To prepare samples for flow cytometric analysis, blood was washed out of methanol fixative and incubated with antibodies and other reagents as specified in the In Vivo Mouse MicroFlow Kit manual. This methodology has been described in detail elsewhere (Dertinger et al. 2004). Kit-supplied malaria-infected erythrocytes served as biologi- cal standards and guided instrument settings on each day of analysis (Tometsko et al. 1993). Micronucleus frequencies were determined upon the acquisition of 20,000 CD71-positive reticulocytes (RET) per blood sample. A BD FACSCalibur flow cytometer running CellQuest Pro v5.2 was used for these analyses. LacZMutation: Sample Preparation,DataAcquisition LacZ mutant frequencies were determined on coded bone marrow sam- ples collected at 28 + 3d and 28 + 70d. Bone marrow total genomic DNA was extracted according to Gingerich et al. (2014). Briefly, bone marrow was thawed, then 50 μL was digested in 5 mL lysis buffer (10 mM EDTA, 100 mM NaCl, 10 mM Tris–HCl, pH 7.6, 1% sodium dodecyl sulfate [w/v] and 1 mg/mL Proteinase K [Invitrogen, Burlington, Canada]), and incubated at 37�C overnight with gentle shaking. Genomic DNA was extracted using phenol/chloroform extraction procedure and was then stored at 4�C in 90 μL TE buffer (10 mM Tris pH 7.6, 0.1 mM EDTA). The P-Gal positive selection assay was used for determining lacZ mutant frequency in DNA samples as previously described (Gingerich et al. 2014). The recombinant λgt10 phage vector was transfected from genomic DNA using Packaging Extract kits then mixed with the host bac- terium (Escherichia coli lacZ−/galE−), plated on medium containing 0.3% (w/v) P-Gal and incubated overnight at 37�C. Mutant frequency was cal- culated as the ratio of mutant plaque forming units (pfu) to total pfu. The DNA from two mice (a control and one middle dose at 28 + 3d) did not pack well and produced no colonies; while two mice (one from the low dose and one from the middle dose) had to be euthanized before tissue col- lection at 28 + 70d because of rectal prolapse. Pig-aMutation: Sample Preparation,DataAcquisition Pig-a mutant phenotype cell frequencies were determined on coded blood samples collected at 28 + 3d and 28 + 70d. Methods for processing mouse blood for Pig-a measurements have been described (Labash et al. 2016). As with previous reports, the frequency of mutant phenotype reticulocytes (RETCD24-) as well as the frequency of mutant phenotype erythrocytes (RBCCD24-) was determined for each sample. An Instrument Calibration Standard was generated on each day data acquisition occurred. These samples contained a high prevalence of mutant-mimic cells and pro- vided a means to define the location of GPI anchor-deficient erythrocytes (Phonethepswath et al. 2010). A BD FACSCanto II flow cytometer run- ning FACSDiva v6.1.1 was used for the Pig-a analyses. Calculations, Statistical Analyses The incidence of MN-RET, MN-NCE, and RET are expressed as fre- quency percent. The formulas used to calculate RETCD24- and RBCCD24- frequencies based on pre- and post-immunomagnetic column data are described by Dertinger et al. (2012) and the MutaFlow manual (www. litronlabs.com). To evaluate the effect that PCH treatment may have had on %MN- RET, %MN-NCE, %RET, and RBCCD24- and RETCD24- frequencies relative to concurrent vehicle control mice, Dunnett’s multiple comparison t-tests were performed using JMP software’s one-way ANOVA platform (v12.0.1, SAS Institute, Cary, NC). With the exception of the %RET end- point, each test was performed at the 5% level using a one-tailed test to identify significant increases relative to vehicle control. In the case of %RET, the tests were two-tailed in order to determine whether a signifi- cant treatment-related increase or decrease occurred. Also, note that as similar frequencies were observed for vehicle control mice across every endpoint and both time points, we pooled these data to achieve a larger negative control group size. Prior to performing the parametric tests described above, Levene’s tests were used to verify the equality of variances in the samples (P > 0.05; JMP’s ANOVA platform). The following transformations were applied to fulfill this requirement: %MN-RET and %MN-NCE values were cube root transformed; and RBCCD24-, RETCD24-, and lacZ mutation frequencies were log10 transformed. Pearson correlation coefficients were calculated for lacZ and Pig-a mean mutant frequencies at the 28 + 3d and 28 + 70d time points (Microsoft Excel for Mac 2008, v12.3.6, Microsoft, Redmond, WA). This was done for both RBCCD24- vs. lacZ mutant frequencies, and RETCD24- vs. lacZ mutant frequencies. RESULTS We evaluated the induction of MN, Pig-a, and lacZ mutations in the hematopoietic system of MutaMouse males at different time points after cessation of a 28-day exposure to PCH. No significant reduction in body weight Environmental and Molecular Mutagenesis. DOI 10.1002/em 507Integrated in vivoGenotoxicity Assessment of PCH http://www.litronlabs.com http://www.litronlabs.com was observed at the end of the 4 weeks of exposure, or at the later time point (data not shown). %RET were measured at 28 + 2d in conjunction with MN-RET analyses. Slightly elevated %RET were observed, with a statistically significant increase evident for the high dose group (Fig. 1A). Higher %RET values that occur sev- eral days after cessation of treatment are generally indica- tive of treatment-induced bone marrow toxicity, with a consequent period of enhanced erythropoiesis activity upon cessation of treatment (Dertinger et al. 2012). Robust, dose-related MN induction was also observed for PCH- exposed mice. Both MN-RET and MN-NCE frequencies exhibited statistically significant increases (P < 0.05), even at the low and middle dose levels that were not observed to appreciably affect erythropoiesis (Fig. 1B,C). For the high dose group, MN-RET, and MN-NCE frequencies exhibited mean fold increases of 9.1x and 6.8x, respectively. As shown by Figure 2A, treatment with PCH for 28 con- secutive days caused dose-related increases in lacZ mutant frequencies at 28 + 3d with significant fold increases of 2.0-, 3.5-, and 6.8-fold at the low, medium, and high doses (P < 0.05 for all doses), respectively. These effects were sustained at 28 + 70d, with fold increases of 1.6-, 2.5-, and 5.0- at the low, medium, and high dose (P < 0.05; Fig. 2B). Although the latter harvest time exhibited modestly lower mean PCH-induced mutant frequencies, and within group variation was more pronounced, these results indicate that PCH induced mutagenicity in long-lived bone marrow pro- genitor cells. Mean lacZ frequencies for each treatment Fig.1. Frequency of Day 28 + 2d blood reticulocytes (A), micronucleated immature erythrocytes (B), and micronucleated mature erythrocytes (C) for each of four PCH treatment groups, where Low = 6.25, Mid = 12.5, and High = 25 mg/kg/day. Data for individual mice are shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by a gray circle as opposed to a red circle. Circles’ diameters represent 95% confidence intervals (A) or 90% confidence intervals (B and C). Fig. 2. Frequency of Day 28 + 3d lacZ mutations (A) and Day 28 + 70d lacZ mutations (B) for each of four PCH treatment groups, where Low = 6.25, Mid = 12.5, and High = 25 mg/kg/day. Data for individual mice are shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by a gray circle as opposed to a red circle. Circles’ diameters represent 90% confidence intervals. Environmental and Molecular Mutagenesis. DOI 10.1002/em 508 Maurice et al. condition and time point are provided in Supporting Infor- mation Table S1. RETCD24- frequencies were significantly increased in PCH-treated mice on days 28 + 3d and 28 + 70d (Fig. 3A, C). RETCD24- responses were more variable at the later time point. As with the lacZ results, the fact that elevated RETCD24- frequencies were observed at the late time point suggests that some portion of the mutated cells arose from long- lived progenitors with extended self-renewal capacity. RBCCD24- frequencies also showed responses to PCH exposure, with statistically significant increases observed in all PCH treatment groups and at both time points (Fig. 3B, D). Note that the increase observed in the low dose group at 28 + 70d must be qualified to some extent because statis- tical significance is lost when the animal with the highest mutant frequency is removed. Even so, the result is not likely a scoring artifact as this animal also had the highest mutant RET value among the animals in the same group. The raw Pig-a data and calculated frequencies, on a per mouse basis, are posted at the Pig-a In Vivo Gene Mutation Assay Database, http://www.pharmacy.umaryland. edu/centers/cersi-files. Pearson correlation coefficients were derived for compar- isons across the two mutation assays. At 28 + 3d, mean RETCD24- and RBCCD24- vs. lacZ frequencies showed particularly high correlations, with R2 values of 0.956 and 0.999, respectively (P < 0.05, Fig. 4A,B). At 28 + 70d, mean RETCD24- were also in good agreement with lacZ (R2 = 0.996, P < 0.05, Figure 4C), while the RBCCD24- correlation was slightly lower (R2 = 0.902, P < 0.05, Figure 4D). DISCUSSION There is currently considerable pressure to transition the practice of toxicology toward computational and in vitro methodologies (Dix et al. 2007; Collins et al. 2008; Kavlock et al. 2009). In light of this, and given its predom- inately negative in vitro DNA-damage profile, PCH made an interesting case study for evaluating multiple in vivo endpoints of genotoxicity—lacZ and Pig-a mutation, and chromosomal damage in the form of MN. By concentrating on bone marrow and blood-based assays, we focused on the hemotopoietic compartment and the notable hematolog- ical secondary cancers that have been described for PCH exposure (Levine and Bloomfield 1992). Furthermore, by using an experimental design that facilitated assessment of all three assays, this PCH study was performed in a manner that addresses resource and animal-usage concerns that are in part driving the transition noted above. Fig. 3. Frequency of 28 + 3d Pig-a mutant reticulocytes (A) and Pig-a mutant erythrocytes (B), as well as 28 + 70d Pig-a mutant reticulocytes (C) and Pig-a mutant erythrocytes (D) for each of four PCH treatment groups, where Low = 6.25, Mid = 12.5, and High = 25 mg/kg/day. Data for individual mice are shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by a gray circle as opposed to a red circle. Circles’ diameters represent 90% confidence intervals. Environmental and Molecular Mutagenesis. DOI 10.1002/em 509Integrated in vivoGenotoxicity Assessment of PCH http://www.pharmacy.umaryland.edu/centers/cersi-files http://www.pharmacy.umaryland.edu/centers/cersi-files In this study, PCH’s in vivo genotoxic activity was read- ily apparent, as each of the endpoints investigated showed statistically significant effects at all of the doses tested. As also found by Suzuki et al. (1999), we observed potent clastogenic activity as evidenced by robust MN induction. Whereas Suzuki and colleagues failed to detect significant increases in in vivo lacZ mutations in bone marrow, liver, testis, spleen, kidney, and lung upon single administration of 50 mg PCH/kg, they did observe significant lacZ muta- tion induction at 150 mg/kg/day when administered for five consecutive days (cumulative dose: 750 mg/kg) partic- ularly in those tissues that are prone to develop tumors fol- lowing PCH exposure (i.e., lung, bone marrow, and spleen). In our experiments, we demonstrated that a strong mutagenic response in the bone marrow is occurring at much lower doses, as even at the lowest PCH dose studied (6.25 mg/kg/day, or a cumulative dose of 175 mg/kg) resulted in significant induction of bone marrow lacZ and blood Pig-a mutation. These results show that PCH elicits a robust mutagenic response in the bone marrow even at doses that do not produce cytotoxicity. While Phonethepswath et al. (2013) also reported PCH-induced Pig-a mutation in mice, it is not productive comparing those results with data reported herein because the previous study utilized an early mouse blood labeling protocol that is now understood to systematically underestimate mutant cell frequency (Labash et al. 2016). Finally, Chen et al. (2019) recently reported strong induction of Pig-a mutations following a 28-day exposure regimen in rats using daily doses higher than those used here. Thus, in addition to its well-known clastogenic activity, PCH is also an effective in vivo mutagen when recommended OECD test guideline experimental design considerations are followed. One of the objectives of our study was to compare the mutagenic response observed with two reporter genes that are routinely used for assessing chemical mutagenesis. We Fig. 4. The mean frequency of Pig-a mutant cells are graphed against the mean frequency of lacZ mutation for each of four dose groups and each of two time points, with Pearson correlation coefficients provided by an R2 value. Error bars represent one standard deviation. A = 28 + 3d results, where Pig-a mutants are reticulocytes; B = 28 + 3d results, Pig-a mutants are erythrocytes; C = 28 + 70d results, Pig-a mutants are reticulocytes; and D = 28 + 70d results, Pig-a mutants are erythrocytes. Environmental and Molecular Mutagenesis. DOI 10.1002/em 510 Maurice et al. found a very strong correlation (R2 ≥ 0.956) between lacZ mutant frequencies in bone marrow and Pig-a mutant RET and RBC at the early time point (i.e., 28 + 3d). At the later time point (i.e., 28 + 70d), the correlation between the two assays was still very strong (R2 = 0.996) when comparing lacZ mutant frequencies with Pig-a mutant RET. The cor- relation was slightly lower between mutant lacZ bone mar- row cells and Pig-a mutant RBC (R2 = 0.902), a result that reinforces expert recommendations to study both RET and RBC cohorts for Pig-a mutation (Gollapudi et al. 2015). Overall, our mutation results are in agreement with those reported by Lemieux and collaborators (Lemieux et al. 2011) who observed a significant, and comparable, dose-related increase in mutant cells with the Pig-a and lacZ assays following exposure of MutaMouse males to benzo[a]pyrene, a strong mutagen that acts through a completely different mode of action compared to PCH. These results support the notion that both assays have a broad applicability domain and that they both are effective methods for evaluating gene mutation events. The use of both gene mutation assays in an integrated test design represents a convenient means to address limita- tions that are generally ascribed to the two assays when used in isolation. The lacZ mutation assay is a well- validated and regulatory-accepted assay with an adopted OECD guideline that can be used for assessing mutagenic- ity in any tissue. A potential limitation of the assay is that it uses a bacterial gene that is highly methylated and not transcribed, and thus may not be fully representative of the susceptibility of endogenous genes to mutation induction (Lambert et al. 2005). It is also detecting almost exclusively point mutations (Beal et al. 2015). On the other hand, the Pig-a gene is an endogenous gene and its inactivation can occur through a variety of mutational events including those that would not be detected by the lacZ assay. How- ever, the Pig-a assay can currently only be conducted in erythropoietic cells and it is challenging to sequence pre- sumed mutant cells to characterize the types of lesions responsible for the mutant phenotype. It should also be noted that an OECD test guideline for the Pig-a assay is currently under development, and the results reported herein add further support to these validation efforts. In summary, our data agree with previous studies that found PCH exerts potent clastogenic activity. Furthermore, detection of its mutagenic potential is also readily observed when more careful analysis, particularly in terms of dosing protocol and harvest times, are followed. The advantage of combining the lacZ, Pig-a, and MN endpoints is that it allows for a comprehensive assessment of multiple types of DNA lesions, a particularly important aspect when study- ing new chemical classes and/or when complex metabolism makes choosing a single in vivo genotoxicity endpoint diffi- cult to choose a priori. By coupling the gene mutation assess- ments with MN analyses, it becomes possible to efficiently detect a chemical’s mutagenic and chromosome damaging potential. Thus, when systemic exposure of a chemical and its reactive intermediate(s) can be verified, this approach rep- resents an efficient and cost-effective means of obtaining valuable safety data that includes mode of action information that has not traditionally been collected due to technical and cost considerations. ACKNOWLEDGMENTS Excellent benchtop work provided by Dorothea Torous, Svetlana Avlasevich, and Carson Labash is greatly appreci- ated. This research was funded through Health Canada’s Chemicals Management Plan. C.M. was funded through the Natural Sciences and Engineering Research Council of Canada’s Visiting Fellowships in a Government Laboratory Program. We are grateful to Karine Chamberland, Julie Todd, Kevin Little, Scott Smith, Julie Cox, Drs. Martha Navarro, Marc Beal, Matthew Meier, Alexandra Long, and Richard Webster for assistance with animal study and necropsy. AUTHORCONTRIBUTIONS F.M., C.Y., S.D. and C.M. designed these studies. 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The in vitro micronucleus test on iso- lated human lymphocytes. Mutat Res 291(1):93–102. Accepted by— B. Gollapudi Environmental and Molecular Mutagenesis. DOI 10.1002/em 512 Maurice et al. Integrated In Vivo Genotoxicity Assessment of Procarbazine Hydrochloride Demonstrates Induction of Pig-a and LacZ Mutation... INTRODUCTION MATERIALS AND METHODS Reagents and Other Supplies Animals, Treatments, Blood Harvests Micronucleated Reticulocytes: Sample Preparation, Data Acquisition LacZ Mutation: Sample Preparation, Data Acquisition Pig-a Mutation: Sample Preparation, Data Acquisition Calculations, Statistical Analyses RESULTS DISCUSSION ACKNOWLEDGMENTS AUTHOR CONTRIBUTIONS DISCLOSURE REFERENCES