In planta proximity dependent biotin identification (BioID)
- Download(s)
- DOI
- Language of the publication
- English
- Date
- 2018-06-15
- Type
- Article
- Author(s)
- Khan, Madiha
- Youn, Ji-Young
- Gingras, Anne-Claude
- Subramaniam, Rajagopal
- Desveaux, Darrell
- Publisher
- Nature Publishing Group
Abstract
Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.
Subject
- Plants
Keywords
- Plant molecular biology
Rights
Pagination
1-8
Peer review
Yes
Open access level
Gold
Identifiers
- Government document number
- 2045-2322
Article
- Journal title
- Scientific Reports
- Journal volume
- 8
- Article number
- 9212
- Accepted date
- 2018-06-04
- Submitted date
- 2018-04-11
Citation(s)
Khan, M., Youn, J.-Y., Gingras, A.-C., Subramaniam, R., & Desveaux, D. (2018). In planta proximity dependent biotin identification (BioID). Scientific Reports, 8, Article 9212. https://doi.org/10.1038/s41598-018-27500-3