Enzyme-linked immunosorbent assay using Henipavirus-Receptor EphrinB2 and monoclonal antibodies for detecting Nipah and Hendra viruses
Enzyme-linked immunosorbent assay using Henipavirus-Receptor EphrinB2 and monoclonal antibodies for detecting Nipah and Hendra viruses
Simple item page
Full item details
- creativework.keywords - en
- Nipah virus
- Monoclonal antibodies
- Zoonotic and emerging pathogens
- Zoonosis
- creativework.keywords - fr
- Virus Nipah
- Anticorps monoclonaux
- Pathogènes zoonotiques et émergents
- Zoonoses
- dc.contributor.author
- Zhu, Wenjun
- Smith, Greg
- Pickering, Bradley
- Banadyga, Logan
- Yang, Ming
- dc.date.accepted
- 2024-05-14
- dc.date.accessioned
- 2025-03-14T17:05:25Z
- dc.date.available
- 2025-03-14T17:05:25Z
- dc.date.issued
- 2024-05-16
- dc.date.submitted
- 2024-03-27
- dc.description.abstract - en
- The Nipah virus (NiV) and the Hendra virus (HeV) are highly pathogenic zoonotic diseases that can cause fatal infections in humans and animals. Early detection is critical for the control of NiV and HeV infections. We present the development of two antigen-detection ELISAs (AgELISAs) using the henipavirus-receptor EphrinB2 and monoclonal antibodies (mAbs) to detect NiV and HeV. The NiV AgELISA detected only NiV, whereas the NiV/HeV AgELISA detected both NiV and HeV. The diagnostic specificities of the NiV AgELISA and the NiV/HeV AgELISA were 100% and 97.8%, respectively. Both assays were specific for henipaviruses and showed no cross-reactivity with other viruses. The AgELISAs detected NiV antigen in experimental pig nasal wash samples taken at 4 days post-infection. With the combination of both AgELISAs, NiV can be differentiated from HeV. Complementing other henipavirus detection methods, these two newly developed AgELISAs can rapidly detect NiV and HeV in a large number of samples and are suitable for use in remote areas where other tests are not available.
- dc.identifier.citation
- Zhu, W., Smith, G., Pickering, B., Banadyga, L., & Yang, M. (2024, May 16). Enzyme-linked immunosorbent assay using henipavirus-receptor ephrinb2 and monoclonal antibodies for detecting Nipah and hendra viruses. MDPI. https://doi.org/10.3390/v16050794
- dc.identifier.doi
- https://doi.org/10.3390/v16050794
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3517
- dc.language.iso
- en
- dc.publisher - en
- MDPI
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Animal diseases
- dc.subject - fr
- Maladie animale
- dc.subject.en - en
- Animal diseases
- dc.subject.fr - fr
- Maladie animale
- dc.title - en
- Enzyme-linked immunosorbent assay using Henipavirus-Receptor EphrinB2 and monoclonal antibodies for detecting Nipah and Hendra viruses
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 794
- local.article.journalissue
- 5
- local.article.journaltitle - en
- Viruses
- local.article.journalvolume
- 16
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
Download(s)
Original bundle
1 - 1 of 1
Name: EnzymeLinkedImmunosorbentAssayHenipavirusReceptorEphrinB2MonoclonalAntibodiesDetectingNipahHendraViruses.pdf
Size: 1.71 MB
Format: PDF
Collection(s)