Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
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- creativework.keywords - en
- plant genetics
- plant immunity
- creativework.keywords - fr
- génétique végétale
- immunité des plantes
- dc.contributor.author
- Hiebert, Colin W.
- Moscou, Matthew J.
- Hewitt, Tim
- Steuernagel, Burkhard
- Hernández-Pinzón, Inma
- Green, Phon
- Pujol, Vincent
- Zhang, Peng
- Rouse, Matthew N.
- Jin, Yue
- McIntosh, Robert A.
- Upadhyaya, Narayana
- Zhang, Jianping
- Bhavani, Sridhar
- Vrána, Jan
- Karafiátová, Miroslava
- Huang, Li
- Fetch, Tom
- Doležel, Jaroslav
- Wulff, Brande B.H.
- Lagudah, Evans
- Spielmeyer, Wolfgang
- dc.date.accessioned
- 2023-04-13T18:03:33Z
- dc.date.available
- 2023-04-13T18:03:33Z
- dc.date.issued
- 2020-02-28
- dc.description.abstract - en
- Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
- dc.identifier.citation
- Hiebert, C. W., Moscou, M. J., Hewitt, T., Steuernagel, B., Hernández-Pinzón, I., Green, P., Pujol, V., Zhang, P., Rouse, M. N., Jin, Y., McIntosh, R. A., Upadhyaya, N., Zhang, J., Bhavani, S., Vrána, J., Karafiátová, M., Huang, L., Fetch, T., Doležel, J., … Spielmeyer, W. (2020). Stem rust resistance in wheat is suppressed by a subunit of the Mediator Complex. Nature Communications, 11(1). https://doi.org/10.1038/s41467-020-14937-2
- dc.identifier.doi
- https://doi.org/10.1038/s41467-020-14937-2
- dc.identifier.issn
- 2041-1723
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/151
- dc.language.iso
- en
- dc.publisher
- Springer Nature
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.subject - en
- Agriculture
- dc.subject - fr
- Agriculture
- dc.subject.en - en
- Agriculture
- dc.subject.fr - fr
- Agriculture
- dc.title - en
- Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
- dc.title.fosrctranslation - fr
- Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journalissue
- 1
- local.article.journaltitle
- Nature Communications
- local.article.journalvolume
- 11
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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