Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture

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DOI

https://doi.org/10.1016/j.jff.2022.105044

Language of the publication
English
Date
2022-04-01
Type
Article
Author(s)
  • Peng, Li
  • Guo, Fanghua
  • Pei, Minjia
  • Tsao, Rong
  • Wang, Xiaoya
  • Jiang, Li
  • Sun, Yong
  • Xiong, Hua
Publisher
Elsevier Ltd.

Abstract

The polyphenol-rich lentil hulls are the by-product of lentils hulling process. In this manuscript, in vitro digestion, Caco-2 cell monolayer and Caco-2/RAW264.7 cell co-culture model were established to explore their anti-inflammatory mechanism, absorption of digestive products (RLD), and impact on the intestinal barrier. Results shown that high dose RLE and GLE could significantly inhibit the secretion of NO (30.23% and 31.08%, respectively), IL-6 (81.48% and 56.82%, respectively) and IL-1β (88.05% and 91.67%, respectively), and down-regulate the protein and mRNA expression of iNOS (56.46% and 45.69%, respectively) and COX-2 (76.53% and 46.65%, respectively), and inhibit the activation of MAPK and NF-κB signaling pathways. Polyphenols can be released from lentil hulls and protocatechuic acid glycoside derivative has the highest content (2205.09 ± 7.02 μg/g DW). Digestive products can be absorbed by intestine to maintain intestinal barrier and play anti-inflammatory effect. Above all, lentil hulls may be a potentially valuable functional dietary resource.

Subject

  • Agriculture

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Peer review

Yes

Open access level

Gold

Identifiers

ISSN
1756-4646

Article

Journal title
Journal of Functional Foods
Journal volume
92
Article number
105044
Accepted date
2022-03-22
Submitted date
2021-12-13

Citation(s)

Peng, L., Guo, F., Pei, M., Tsao, R., Wang, X., Jiang, L., Sun, Y., & Xiong, H. (2022). Anti-inflammatory effect of lentil hull (Lens Culinaris) extract via MAPK/NF-ΚB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture. Journal of Functional Foods, 92, 105044. https://doi.org/10.1016/j.jff.2022.105044

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Food and beverages

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