Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture
Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture
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- dc.contributor.author
- Peng, Li
- Guo, Fanghua
- Pei, Minjia
- Tsao, Rong
- Wang, Xiaoya
- Jiang, Li
- Sun, Yong
- Xiong, Hua
- dc.date.accepted
- 2022-03-22
- dc.date.accessioned
- 2024-07-16T20:19:02Z
- dc.date.available
- 2024-07-16T20:19:02Z
- dc.date.issued
- 2022-04-01
- dc.date.submitted
- 2021-12-13
- dc.description.abstract - en
- The polyphenol-rich lentil hulls are the by-product of lentils hulling process. In this manuscript, in vitro digestion, Caco-2 cell monolayer and Caco-2/RAW264.7 cell co-culture model were established to explore their anti-inflammatory mechanism, absorption of digestive products (RLD), and impact on the intestinal barrier. Results shown that high dose RLE and GLE could significantly inhibit the secretion of NO (30.23% and 31.08%, respectively), IL-6 (81.48% and 56.82%, respectively) and IL-1β (88.05% and 91.67%, respectively), and down-regulate the protein and mRNA expression of iNOS (56.46% and 45.69%, respectively) and COX-2 (76.53% and 46.65%, respectively), and inhibit the activation of MAPK and NF-κB signaling pathways. Polyphenols can be released from lentil hulls and protocatechuic acid glycoside derivative has the highest content (2205.09 ± 7.02 μg/g DW). Digestive products can be absorbed by intestine to maintain intestinal barrier and play anti-inflammatory effect. Above all, lentil hulls may be a potentially valuable functional dietary resource.
- dc.identifier.citation
- Peng, L., Guo, F., Pei, M., Tsao, R., Wang, X., Jiang, L., Sun, Y., & Xiong, H. (2022). Anti-inflammatory effect of lentil hull (Lens Culinaris) extract via MAPK/NF-ΚB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture. Journal of Functional Foods, 92, 105044. https://doi.org/10.1016/j.jff.2022.105044
- dc.identifier.doi
- https://doi.org/10.1016/j.jff.2022.105044
- dc.identifier.issn
- 1756-4646
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/2700
- dc.language.iso
- en
- dc.publisher
- Elsevier Ltd.
- dc.rights - en
- Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
- dc.rights - fr
- Creative Commons Attribution - Pas d'utilisation commerciale - Pas de modification 4.0 International (CC BY-NC-ND 4.0)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by-nc-nd/4.0/deed.fr
- dc.subject - en
- Agriculture
- dc.subject - fr
- Agriculture
- dc.subject.en - en
- Agriculture
- dc.subject.fr - fr
- Agriculture
- dc.title - en
- Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 105044
- local.article.journaltitle
- Journal of Functional Foods
- local.article.journalvolume
- 92
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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